Apple receptor-like kinase FERONIA regulates salt tolerance and ABA sensitivity in Malus domestica
Copyright © 2022 Elsevier GmbH. All rights reserved.
| Veröffentlicht in: | Journal of plant physiology. - 1979. - 270(2022) vom: 12. März, Seite 153616 |
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| 1. Verfasser: | |
| Weitere Verfasser: | , , , , , , , |
| Format: | Online-Aufsatz |
| Sprache: | English |
| Veröffentlicht: |
2022
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| Zugriff auf das übergeordnete Werk: | Journal of plant physiology |
| Schlagworte: | Journal Article ABA Apple MdFER ROS Receptor-like kinase protein FERONIA Salinity |
| Zusammenfassung: | Copyright © 2022 Elsevier GmbH. All rights reserved. FERONIA (FER) is a membrane-localized receptor-like kinase that plays pivotal roles in male and female gametophyte recognition, hormone signaling crosstalk, and biotic and abiotic responses. Most reports focus on the functions of FER in model plant Arabidopsis thaliana. However, the functions of FER homologs have not been deeply investigated in apple (Malus domestica), an important economic fruit crop distributed worldwide, especially in China. In this study, we identified an apple homolog of Arabidopsis FER, named MdFER (MDP0000390677). The two proteins encoded by AtFER and MdFER share similar domains: an extracellular malectin-like domain, a transmembrane domain, and an intracellular kinase domain. MdFER was further proven to localize to the plasma membrane in the epidermal cells of Nicotiana benthamiana. MdFER was widely expressed in different apple tissues, but the highest expression was found in roots. In addition, expression of MdFER was significantly induced by treatment with abscisic acid (ABA) and salt (NaCl). Overexpressing MdFER dramatically improved the resistance to salt stress and reduced the sensitivity to ABA in apple callus, while suppressing MdFER expression showed contrary effects. Furthermore, ectopic expression of MdFER in Arabidopsis significantly increased the salt tolerance and reduced the sensitivity to ABA. In addition, under salt stress and ABA treatment, Arabidopsis with highly expressed MdFER accumulated less reactive oxygen species (ROS), and the enzymatic activity of two ROS scavengers, superoxide dismutase and catalase, was higher compared with that of wild type (WT). Our work proves that MdFER positively regulates salt tolerance and negatively regulates ABA sensitivity in apple, which enriched the functions of FER in different plant species |
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| Beschreibung: | Date Revised 20.10.2023 published: Print-Electronic Citation Status PubMed-not-MEDLINE |
| ISSN: | 1618-1328 |
| DOI: | 10.1016/j.jplph.2022.153616 |