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231225s2022 xx |||||o 00| ||eng c |
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|a 10.1111/nph.17957
|2 doi
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|a pubmed24n1118.xml
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|a DE-627
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|e rakwb
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|a eng
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|a Wieloch, Thomas
|e verfasserin
|4 aut
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|a Anaplerotic flux into the Calvin-Benson cycle
|b hydrogen isotope evidence for in vivo occurrence in C3 metabolism
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|c 2022
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
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|2 rdamedia
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|a ƒa Online-Ressource
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|2 rdacarrier
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|a Date Completed 31.03.2022
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|a Date Revised 31.07.2022
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a © 2022 The Authors New Phytologist © 2022 New Phytologist Foundation.
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|a As the central carbon uptake pathway in photosynthetic cells, the Calvin-Benson cycle is among the most important biochemical cycles for life on Earth. A carbon flux of anaplerotic origin (i.e. through the chloroplast-localized oxidative branch of the pentose phosphate pathway) into the Calvin-Benson cycle was proposed recently. Here, we measured intramolecular deuterium abundances in leaf starch of Helianthus annuus grown at varying ambient CO2 concentrations, Ca . Additionally, we modelled deuterium fractionations expected for the anaplerotic pathway and compared modelled with measured fractionations. We report deuterium fractionation signals at H1 and H2 of starch glucose. Below a Ca change point, these signals increase with decreasing Ca consistent with modelled fractionations by anaplerotic flux. Under standard conditions (Ca = 450 ppm corresponding to intercellular CO2 concentrations, Ci , of 328 ppm), we estimate negligible anaplerotic flux. At Ca = 180 ppm (Ci = 140 ppm), more than 10% of the glucose-6-phosphate entering the starch biosynthesis pathway is diverted into the anaplerotic pathway. In conclusion, we report evidence consistent with anaplerotic carbon flux into the Calvin-Benson cycle in vivo. We propose the flux may help to: maintain high levels of ribulose 1,5-bisphosphate under source-limited growth conditions to facilitate photorespiratory nitrogen assimilation required to build-up source strength; and counteract oxidative stress
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|a Journal Article
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|a Calvin-Benson cycle
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|a flux estimation
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|a glucose-6-phosphate shunt
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|a hydrogen stable isotopes
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|a nitrogen assimilation
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|a oxidative pentose phosphate pathway
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| 650 |
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|a plant carbon metabolism
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|a source-sink balance
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|a Isotopes
|2 NLM
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|a Hydrogen
|2 NLM
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|a 7YNJ3PO35Z
|2 NLM
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| 700 |
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|a Augusti, Angela
|e verfasserin
|4 aut
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|a Schleucher, Jürgen
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t The New phytologist
|d 1979
|g 234(2022), 2 vom: 12. Apr., Seite 405-411
|w (DE-627)NLM09818248X
|x 1469-8137
|7 nnns
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| 773 |
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|g volume:234
|g year:2022
|g number:2
|g day:12
|g month:04
|g pages:405-411
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|u http://dx.doi.org/10.1111/nph.17957
|3 Volltext
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