Matrilineal empowers wheat pollen with haploid induction potency by triggering postmitosis reactive oxygen species activity

Matrilineal empowers wheat pollen with haploid induction potency by triggering postmitosis reactive oxygen species activity

© 2022 The Authors. New Phytologist © 2022 New Phytologist Foundation.

Détails bibliographiques
Publié dans:The New phytologist. - 1984. - 233(2022), 6 vom: 19. März, Seite 2405-2414
Auteur principal: Sun, Guoliang (Auteur)
Autres auteurs: Geng, Shuaifeng, Zhang, Hongjie, Jia, Meiling, Wang, Zhenyu, Deng, Zhongyin, Tao, Shu, Liao, Ruyi, Wang, Fang, Kong, Xingchen, Fu, Mingxue, Liu, Shaoshuai, Li, Aili, Mao, Long
Format: Article en ligne
Langue:English
Publié: 2022
Accès à la collection:The New phytologist
Sujets:Journal Article Research Support, Non-U.S. Gov't DNA damage double haploid haploid induction reactive oxygen species (ROS) wheat Reactive Oxygen Species
Description
Résumé:© 2022 The Authors. New Phytologist © 2022 New Phytologist Foundation.
Reactive oxygen species (ROS) play important roles during anther and pollen development. DNA damage may cause chromosome fragmentation that is considered to underlie chromosome elimination for haploid induction by matrilineal pollen, a key step in MATRILINEAL-based double haploid breeding technology. But when and how DNA damage occurs is unknown. We performed comparative studies of wheat pollens from the wild-type and the CRISPR/Cas9 edited matrilineal mutant (mMTL). Chemical assays detected a second wave of ROS in mMTL pollen at the three-nuclei-stage and subsequently, along with reduced antioxidant enzyme activities. RNA-seq analysis revealed disturbed expression of genes for fatty acid biosynthesis and ROS homoeostasis. Gas chromatography-mass spectrometry measurement identified abnormal fatty acid metabolism that may contribute to defective mMTL pollen walls as observed using electron microscopy, consistent with the function of MTL as a phospholipase. Moreover, DNA damage was identified using TdT-mediated dUTP nick-end labelling and quantified using comet assays. Velocity patterns showed that ROS increments preceded that of DNA damage over the course of pollen maturation. Our work hypothesises that mMTL-triggered later-stage-specific ROS causes DNA damage that may contribute to chromosome fragmentation and hence chromosome elimination during haploid induction. These findings may provide more ways to accelerate double haploid-based plant breeding
Description:Date Completed 31.03.2022
Date Revised 01.04.2022
published: Print-Electronic
Citation Status MEDLINE
ISSN:1469-8137
DOI:10.1111/nph.17963