An aspartic protease 47 causes quantitative recessive resistance to rice black-streaked dwarf virus disease and southern rice black-streaked dwarf virus disease

An aspartic protease 47 causes quantitative recessive resistance to rice black-streaked dwarf virus disease and southern rice black-streaked dwarf virus disease

© 2022 The Authors. New Phytologist © 2022 New Phytologist Foundation.

Bibliographische Detailangaben
Veröffentlicht in:The New phytologist. - 1979. - 233(2022), 6 vom: 19. März, Seite 2520-2533
1. Verfasser: Wang, Zhaoyun (VerfasserIn)
Weitere Verfasser: Zhou, Lian, Lan, Ying, Li, Xuejuan, Wang, Jian, Dong, Jingfang, Guo, Wei, Jing, Dedao, Liu, Qing, Zhang, Shaohong, Liu, Zhiyang, Shi, Wenjuan, Yang, Wu, Yang, Tifeng, Sun, Feng, Du, Linlin, Fu, Hua, Ma, Yamei, Shao, Yudong, Chen, Luo, Li, Jitong, Li, Shuo, Fan, Yongjian, Wang, Yunyue, Leung, Hei, Liu, Bin, Zhou, Yijun, Zhao, Junliang, Zhou, Tong
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2022
Zugriff auf das übergeordnete Werk:The New phytologist
Schlagworte:Journal Article aspartic proteinase quantitative trait loci (QTLs) resistance variety rice black-streaked dwarf virus (RBSDV) southern rice black-streaked dwarf virus (SRBSDV) Aspartic Acid Proteases EC 3.4.- Peptide Hydrolases
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520 |a © 2022 The Authors. New Phytologist © 2022 New Phytologist Foundation. 
520 |a Rice black-streaked dwarf virus disease (RBSDVD) and southern rice black-streaked dwarf virus disease (SRBSDVD) are the most destructive viral diseases in rice. Progress is limited in breeding due to lack of resistance resource and inadequate knowledge on the underlying functional gene. Using genome-wide association study (GWAS), linkage disequilibrium (LD) decay analyses, RNA-sequencing, and genome editing, we identified a highly RBSDVD-resistant variety and its first functional gene. A highly RBSDVD-resistant variety W44 was identified through extensive evaluation of a diverse international rice panel. Seventeen quantitative trait loci (QTLs) were identified among which qRBSDV6-1 had the largest phenotypic effect. It was finely mapped to a 0.8-1.2 Mb region on chromosome 6, with 62 annotated genes. Analysis of the candidate genes underlying qRBSDV6-1 showed high expression of aspartic proteinase 47 (OsAP47) in a susceptible variety, W122, and a low resistance variety, W44. OsAP47 overexpressing lines exhibited significantly reduced resistance, while the knockout mutants exhibited significantly reduced SRBSDVD and RBSDVD severity. Furthermore, the resistant allele Hap1 of OsAP47 is almost exclusive to Indica, but rare in Japonica. Results suggest that OsAP47 knockout by editing is effective for improving RBSDVD and SRBSDVD resistance. This study provides genetic information for breeding resistant cultivars 
650 4 |a Journal Article 
650 4 |a aspartic proteinase 
650 4 |a quantitative trait loci (QTLs) 
650 4 |a resistance variety 
650 4 |a rice black-streaked dwarf virus (RBSDV) 
650 4 |a southern rice black-streaked dwarf virus (SRBSDV) 
650 7 |a Aspartic Acid Proteases  |2 NLM 
650 7 |a EC 3.4.-  |2 NLM 
650 7 |a Peptide Hydrolases  |2 NLM 
650 7 |a EC 3.4.-  |2 NLM 
700 1 |a Zhou, Lian  |e verfasserin  |4 aut 
700 1 |a Lan, Ying  |e verfasserin  |4 aut 
700 1 |a Li, Xuejuan  |e verfasserin  |4 aut 
700 1 |a Wang, Jian  |e verfasserin  |4 aut 
700 1 |a Dong, Jingfang  |e verfasserin  |4 aut 
700 1 |a Guo, Wei  |e verfasserin  |4 aut 
700 1 |a Jing, Dedao  |e verfasserin  |4 aut 
700 1 |a Liu, Qing  |e verfasserin  |4 aut 
700 1 |a Zhang, Shaohong  |e verfasserin  |4 aut 
700 1 |a Liu, Zhiyang  |e verfasserin  |4 aut 
700 1 |a Shi, Wenjuan  |e verfasserin  |4 aut 
700 1 |a Yang, Wu  |e verfasserin  |4 aut 
700 1 |a Yang, Tifeng  |e verfasserin  |4 aut 
700 1 |a Sun, Feng  |e verfasserin  |4 aut 
700 1 |a Du, Linlin  |e verfasserin  |4 aut 
700 1 |a Fu, Hua  |e verfasserin  |4 aut 
700 1 |a Ma, Yamei  |e verfasserin  |4 aut 
700 1 |a Shao, Yudong  |e verfasserin  |4 aut 
700 1 |a Chen, Luo  |e verfasserin  |4 aut 
700 1 |a Li, Jitong  |e verfasserin  |4 aut 
700 1 |a Li, Shuo  |e verfasserin  |4 aut 
700 1 |a Fan, Yongjian  |e verfasserin  |4 aut 
700 1 |a Wang, Yunyue  |e verfasserin  |4 aut 
700 1 |a Leung, Hei  |e verfasserin  |4 aut 
700 1 |a Liu, Bin  |e verfasserin  |4 aut 
700 1 |a Zhou, Yijun  |e verfasserin  |4 aut 
700 1 |a Zhao, Junliang  |e verfasserin  |4 aut 
700 1 |a Zhou, Tong  |e verfasserin  |4 aut 
773 0 8 |i Enthalten in  |t The New phytologist  |d 1979  |g 233(2022), 6 vom: 19. März, Seite 2520-2533  |w (DE-627)NLM09818248X  |x 1469-8137  |7 nnns 
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