Effect of lactic acid addition to equine whole blood on platelet aggregation measured by impedance aggregometry
© 2022 American Society for Veterinary Clinical Pathology.
| Publié dans: | Veterinary clinical pathology. - 1975. - 51(2022), 1 vom: 07. März, Seite 65-69 |
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| Auteur principal: | |
| Autres auteurs: | , , |
| Format: | Article en ligne |
| Langue: | English |
| Publié: |
2022
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| Accès à la collection: | Veterinary clinical pathology |
| Sujets: | Journal Article acidemia aggregometry horse hyperlactatemia platelet function Lactic Acid 33X04XA5AT |
| Résumé: | © 2022 American Society for Veterinary Clinical Pathology. BACKGROUND: Acidemia in sick or injured horses is often due to lactic acid accumulation. Alterations in platelet function and hemostasis are among numerous deleterious effects caused by decreased physiologic pH OBJECTIVES: We aimed to evaluate the effect of hyperlactatemia and resultant acidemia on platelet aggregation in equine whole blood using impedance aggregometry METHODS: Platelet aggregation was measured using the Multiplate analyzer in whole blood from 34 healthy horses at baseline and after in vitro addition of lactic acid to adjust the pH. Platelet aggregation of each sample was quantified by the area under the curve measurement reported by the Multiplate system. The association between platelet aggregation and pH was analyzed using a linear mixed-effects model. The association of baseline platelet aggregation with hematocrits (Hcts), white blood cell (WBC) counts, and platelet counts was evaluated using Pearson's correlations RESULTS: There was a significant association between acidemia and decreased platelet aggregation. No significant correlations were detected between platelet aggregation and Hct, WBC count, or platelet count. Platelet aggregation measured in healthy horses using the Multiplate analyzer showed substantial variation between animals CONCLUSIONS: Acidemia caused by the addition of lactic acid to equine whole blood was associated with a mild though statistically significant decrease in platelet aggregation. In conjunction with other factors, this change may contribute to morbidity-related disorders of hemostasis, although its precise clinical relevance is uncertain |
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| Description: | Date Completed 28.03.2022 Date Revised 01.04.2022 published: Print-Electronic Citation Status MEDLINE |
| ISSN: | 1939-165X |
| DOI: | 10.1111/vcp.13034 |