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|a 10.1093/jxb/erab504
|2 doi
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|a pubmed24n1117.xml
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|a (DE-627)NLM335182542
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|a (NLM)34982825
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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|a Wattelet-Boyer, Valérie
|e verfasserin
|4 aut
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|a Lysophosphatidic acid acyltransferases
|b a link with intracellular protein trafficking in Arabidopsis root cells?
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|c 2022
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
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|2 rdamedia
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|a ƒa Online-Ressource
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|2 rdacarrier
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|a Date Completed 10.03.2022
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|a Date Revised 11.03.2022
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|a published: Print
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|a Citation Status MEDLINE
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|a © The Author(s) 2021. Published by Oxford University Press on behalf of the Society for Experimental Biology. All rights reserved. For permissions, please email: journals.permissionsoup.com.
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|a Phosphatidic acid (PA) and lysophosphatidic acid acyltransferases (LPAATs) might be critical for the secretory pathway. Four extra-plastidial LPAATs (LPAAT2, 3, 4, and 5) were identified in Arabidopsis thaliana. These AtLPAATs display a specific enzymatic activity converting lysophosphatidic acid to PA and are located in the endomembrane system. We investigate a putative role for AtLPAATs 3, 4, and 5 in the secretory pathway of root cells through genetical (knockout mutants), biochemical (activity inhibitor, lipid analyses), and imaging (live and immuno-confocal microscopy) approaches. Treating a lpaat4;lpaat5 double mutant with the LPAAT inhibitor CI976 produced a significant decrease in primary root growth. The trafficking of the auxin transporter PIN2 was disturbed in this lpaat4;lpaat5 double mutant treated with CI976, whereas trafficking of H+-ATPases was unaffected. The lpaat4;lpaat5 double mutant is sensitive to salt stress, and the trafficking of the aquaporin PIP2;7 to the plasma membrane in the lpaat4;lpaat5 double mutant treated with CI976 was reduced. We measured the amounts of neo-synthesized PA in roots, and found a decrease in PA only in the lpaat4;lpaat5 double mutant treated with CI976, suggesting that the protein trafficking impairment was due to a critical PA concentration threshold
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a Arabidopsis
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|a PIN2
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|a PIP2;7
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|a lysophosphatidic acid
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|a lysophosphatidic acid acyltransferase
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|a phosphatidic acid
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|a roots
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|a secretory pathway
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|a Arabidopsis Proteins
|2 NLM
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|a Indoleacetic Acids
|2 NLM
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|a Acyltransferases
|2 NLM
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|a EC 2.3.-
|2 NLM
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|a 2-acylglycerophosphate acyltransferase
|2 NLM
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|a EC 2.3.1.52
|2 NLM
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1 |
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|a Le Guédard, Marina
|e verfasserin
|4 aut
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|a Dittrich-Domergue, Franziska
|e verfasserin
|4 aut
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|a Maneta-Peyret, Lilly
|e verfasserin
|4 aut
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|a Kriechbaumer, Verena
|e verfasserin
|4 aut
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|a Boutté, Yohann
|e verfasserin
|4 aut
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|a Bessoule, Jean-Jacques
|e verfasserin
|4 aut
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|a Moreau, Patrick
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Journal of experimental botany
|d 1985
|g 73(2022), 5 vom: 02. März, Seite 1327-1343
|w (DE-627)NLM098182706
|x 1460-2431
|7 nnns
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|g volume:73
|g year:2022
|g number:5
|g day:02
|g month:03
|g pages:1327-1343
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|u http://dx.doi.org/10.1093/jxb/erab504
|3 Volltext
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|d 73
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|h 1327-1343
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