Rapid Identification of DNA Fragments through Direct Sequencing with Electro-Optical Zero-Mode Waveguides

Rapid Identification of DNA Fragments through Direct Sequencing with Electro-Optical Zero-Mode Waveguides

© 2022 Wiley-VCH GmbH.

Bibliographische Detailangaben
Veröffentlicht in:Advanced materials (Deerfield Beach, Fla.). - 1998. - 34(2022), 9 vom: 01. März, Seite e2108479
1. Verfasser: Farhangdoust, Fatemeh (VerfasserIn)
Weitere Verfasser: Cheng, Feng, Liang, Wentao, Liu, Yongmin, Wanunu, Meni
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2022
Zugriff auf das übergeordnete Werk:Advanced materials (Deerfield Beach, Fla.)
Schlagworte:Journal Article rapid DNA identification single-molecule sequencing zero-mode waveguides DNA 9007-49-2
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500 |a ErratumIn: Adv Mater. 2022 Dec;34(49):e2209376. doi: 10.1002/adma.202209376. - PMID 36482018 
500 |a Citation Status MEDLINE 
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520 |a In contrast to sequence-specific techniques such as polymerase chain reaction, DNA sequencing does not require prior knowledge of the sample for surveying DNA. However, current sequencing technologies demand high inputs for a suitable library preparation, which typically necessitates DNA amplification, even for single-molecule sequencing methods. Here, electro-optical zero-mode waveguides (eZMWs) are presented, which can load DNA into the confinement of zero-mode waveguides with high efficiency and negligible DNA fragment length bias. Using eZMWs, highly efficient voltage-induced loading of DNA fragments of various sizes from ultralow inputs (nanogram-to-picogram levels) is observed. Rapid DNA fragment identification is demonstrated by burst sequencing of short and long DNA molecules (260 and 20 000 bp) loaded from an equimolar picomolar-level concentration mixture in just a few minutes. The device allows further studies in which low-input DNA capture is essential, for example, in epigenetics, where native DNA is required for obtaining modified base information 
650 4 |a Journal Article 
650 4 |a rapid DNA identification 
650 4 |a single-molecule sequencing 
650 4 |a zero-mode waveguides 
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700 1 |a Cheng, Feng  |e verfasserin  |4 aut 
700 1 |a Liang, Wentao  |e verfasserin  |4 aut 
700 1 |a Liu, Yongmin  |e verfasserin  |4 aut 
700 1 |a Wanunu, Meni  |e verfasserin  |4 aut 
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773 1 8 |g volume:34  |g year:2022  |g number:9  |g day:01  |g month:03  |g pages:e2108479 
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