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231225s2022 xx |||||o 00| ||eng c |
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|a 10.1111/nph.17941
|2 doi
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|a pubmed25n1115.xml
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|e rakwb
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|a eng
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|a Li, Shipeng
|e verfasserin
|4 aut
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| 245 |
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|a Arabidopsis ACYL-ACTIVATING ENZYME 9 (AAE9) encoding an isobutyl-CoA synthetase is a key factor connecting branched-chain amino acid catabolism with iso-branched wax biosynthesis
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|c 2022
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
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|2 rdamedia
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|a ƒa Online-Ressource
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|2 rdacarrier
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|a Date Completed 31.03.2022
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|a Date Revised 31.05.2022
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a © 2021 The Authors. New Phytologist © 2021 New Phytologist Foundation.
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|a Iso-branched wax compounds are well known in plants, but their biosynthetic pathways are still mostly unknown. It has been speculated that branched waxes are derived from branched-chain amino acid (BCAA) catabolism, but the evidence for this is very limited. Gas chromatography-flame ionisation detection (GC-FID) analysis revealed that mutations in two subunits of the branched-chain ketoacid dehydrogenase (BCKDH) complex, a key enzyme complex in the degradation of BCAAs, significantly decreased the amounts of branched wax compounds, indicating that BCAA degradation may be integral to the synthesis of iso-branched wax. Substrate feeding studies further revealed that the metabolic precursor of iso-branched wax compounds is isobutyric acid (iBA), which is derived from valine degradation in Arabidopsis. We also isolated a novel mutant and found that its branched wax deficient phenotype could not be rescued by iBA. Map-based cloning together with complementation analysis revealed that mutation in ACYL-ACTIVATING ENZYME 9 (AAE9) is responsible for this phenotype. Genetic and enzyme activity analysis demonstrated that AAE9 is located downstream of the BCAA degradation pathway, and that it activates iBA to isobutyryl-CoA for use on branched wax synthesis. Taken together, our study demonstrates that AAE9 is a key factor connecting BCAA catabolism with branched wax biosynthesis
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a Arabidopsis
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|a acyl-activating enzyme
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|a branched-chain amino acid
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|a iso-branched wax
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|a isobutyric acid
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|a Amino Acids, Branched-Chain
|2 NLM
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|a Arabidopsis Proteins
|2 NLM
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| 650 |
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|a Waxes
|2 NLM
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|a Coenzyme A Ligases
|2 NLM
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| 650 |
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|a EC 6.2.1.-
|2 NLM
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| 700 |
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|a Yang, Xianpeng
|e verfasserin
|4 aut
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| 700 |
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|a Huang, Haodong
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Qiao, Rong
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Jenks, Matthew A
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Zhao, Huayan
|e verfasserin
|4 aut
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| 700 |
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|a Lü, Shiyou
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t The New phytologist
|d 1984
|g 233(2022), 6 vom: 04. März, Seite 2458-2470
|w (DE-627)NLM09818248X
|x 1469-8137
|7 nnas
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| 773 |
1 |
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|g volume:233
|g year:2022
|g number:6
|g day:04
|g month:03
|g pages:2458-2470
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|u http://dx.doi.org/10.1111/nph.17941
|3 Volltext
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