An oomycete effector targets a plant RNA helicase involved in root development and defense

An oomycete effector targets a plant RNA helicase involved in root development and defense

© 2021 The Authors. New Phytologist © 2021 New Phytologist Foundation.

Bibliographische Detailangaben
Veröffentlicht in:The New phytologist. - 1979. - 233(2022), 5 vom: 01. März, Seite 2232-2248
1. Verfasser: Camborde, Laurent (VerfasserIn)
Weitere Verfasser: Kiselev, Andrei, Pel, Michiel J C, Le Ru, Aurélie, Jauneau, Alain, Pouzet, Cécile, Dumas, Bernard, Gaulin, Elodie
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2022
Zugriff auf das übergeordnete Werk:The New phytologist
Schlagworte:Journal Article Research Support, Non-U.S. Gov't Aphanomyces Medicago MtRH10 RNA-helicase effectors nucleolar stress oomycete plant development mehr... Plant Proteins RNA, Plant RNA Helicases EC 3.6.4.13
Beschreibung
Zusammenfassung:© 2021 The Authors. New Phytologist © 2021 New Phytologist Foundation.
Oomycete plant pathogens secrete effector proteins to promote disease. The damaging soilborne legume pathogen Aphanomyces euteiches harbors a specific repertoire of Small Secreted Protein effectors (AeSSPs), but their biological functions remain unknown. Here we characterize AeSSP1256. The function of AeSSP1256 is investigated by physiological and molecular characterization of Medicago truncatula roots expressing the effector. A potential protein target of AeSSP1256 is identified by yeast-two hybrid, co-immunoprecipitation, and fluorescent resonance energy transfer-fluorescence lifetime imaging microscopy (FRET-FLIM) assays, as well as promoter studies and mutant characterization. AeSSP1256 impairs M. truncatula root development and promotes pathogen infection. The effector is localized to the nucleoli rim, triggers nucleoli enlargement and downregulates expression of M. truncatula ribosome-related genes. AeSSP1256 interacts with a functional nucleocytoplasmic plant RNA helicase (MtRH10). AeSSP1256 relocates MtRH10 to the perinucleolar space and hinders its binding to plant RNA. MtRH10 is associated with ribosome-related genes, root development and defense. This work reveals that an oomycete effector targets a plant RNA helicase, possibly to trigger nucleolar stress and thereby promote pathogen infection
Beschreibung:Date Completed 24.03.2022
Date Revised 24.03.2022
published: Print-Electronic
Citation Status MEDLINE
ISSN:1469-8137
DOI:10.1111/nph.17918