CsKDO is a candidate gene regulating seed germination lethality in cucumber
Copyright © 2021 by JAPANESE SOCIETY OF BREEDINGCopyright © 2021 by JAPANESE SOCIETY OF BREEDING.
| Veröffentlicht in: | Breeding science. - 1998. - 71(2021), 4 vom: 15. Sept., Seite 417-425 |
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| 1. Verfasser: | |
| Weitere Verfasser: | , , , , |
| Format: | Online-Aufsatz |
| Sprache: | English |
| Veröffentlicht: |
2021
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| Zugriff auf das übergeordnete Werk: | Breeding science |
| Schlagworte: | Journal Article EMS mutation MutMap+ cucumber genotyping seed germination |
| Zusammenfassung: | Copyright © 2021 by JAPANESE SOCIETY OF BREEDINGCopyright © 2021 by JAPANESE SOCIETY OF BREEDING. Seed germination plays an important role in the initial stage of plant growth. However, few related studies focused on lethality after seed germination in plants. In this study, we identified an Ethyl methanesulfonate (EMS) mutagenesis mutant Csleth with abnormal seed germination in cucumber (Cucumis sativus L.). The radicle of the Csleth mutant grew slowly and detached from the cotyledon until 14 d after sowing. Genetic analysis showed that the mutant phenotype of Csleth was controlled by a single recessive gene. MutMap+ and Kompetitive Allele Specific PCR (KASP) genotyping results demonstrated that Csa3G104930 encoding 3-deoxy-manno-octulosonate cytidylyltransferase (CsKDO) was the candidate gene of the Csleth mutant. The transition mutation of aspartate occurred in Csa3G104930 co-segregated with the phenotyping data. CsKDO was highly expressed in male flowers in wild type cucumbers. Subcellular localization results showed that CsKDO was located in the nucleus. Overall, these results suggest CsKDO regulates lethality during seed germination in cucumber |
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| Beschreibung: | Date Revised 17.12.2021 published: Print-Electronic Citation Status PubMed-not-MEDLINE |
| ISSN: | 1344-7610 |
| DOI: | 10.1270/jsbbs.20149 |