Protein turnover in the developing Triticum aestivum grain

Détails bibliographiques
Publié dans:	The New phytologist. - 1979. - 233(2022), 3 vom: 05. Feb., Seite 1188-1201
Auteur principal:	Cao, Hui (Auteur)
Autres auteurs:	Duncan, Owen, Millar, A Harvey
Format:	Article en ligne
Langue:	English
Publié:	2022
Accès à la collection:	The New phytologist
Sujets:	Journal Article Research Support, Non-U.S. Gov't protein degradation protein synthesis costs protein turnover storage proteins wheat grain Plant Proteins Proteome

Description
Résumé:	© 2021 The Authors. New Phytologist © 2021 New Phytologist Foundation. Protein abundance in cereal grains is determined by the relative rates of protein synthesis and protein degradation during grain development but quantitation of these rates is lacking. Through combining in vivo stable isotope labelling and in-depth quantitative proteomics, we have measured the turnover of 1400 different types of proteins during wheat grain development. We demonstrate that there is a spatiotemporal pattern to protein turnover rates which explain part of the variation in protein abundances that is not attributable to differences in wheat gene expression. We show that c. 20% of total grain adenosine triphosphate (ATP) production is used for grain proteome biogenesis and maintenance, and nearly half of this budget is invested exclusively in storage protein synthesis. We calculate that 25% of newly synthesized storage proteins are turned over during grain development rather than stored. This approach to measure protein turnover rates at proteome scale reveals how different functional categories of grain proteins accumulate, calculates the costs of protein turnover during wheat grain development and identifies the most and the least stable proteins in the developing wheat grain
Description:	Date Completed 24.03.2022 Date Revised 31.07.2022 published: Print-Electronic Citation Status MEDLINE
ISSN:	1469-8137
DOI:	10.1111/nph.17756