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231225s2022 xx |||||o 00| ||eng c |
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|a 10.1002/adma.202106709
|2 doi
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|a eng
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| 100 |
1 |
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|a Abele, Tobias
|e verfasserin
|4 aut
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| 245 |
1 |
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|a Two-Photon 3D Laser Printing Inside Synthetic Cells
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|c 2022
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|a Text
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|a ƒaComputermedien
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|a ƒa Online-Ressource
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|a Date Completed 31.03.2022
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|a Date Revised 01.04.2022
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a © 2021 The Authors. Advanced Materials published by Wiley-VCH GmbH.
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|a Toward the ambitious goal of manufacturing synthetic cells from the bottom up, various cellular components have already been reconstituted inside lipid vesicles. However, the deterministic positioning of these components inside the compartment has remained elusive. Here, by using two-photon 3D laser printing, 2D and 3D hydrogel architectures are manufactured with high precision and nearly arbitrary shape inside preformed giant unilamellar lipid vesicles (GUVs). The required water-soluble photoresist is brought into the GUVs by diffusion in a single mixing step. Crucially, femtosecond two-photon printing inside the compartment does not destroy the GUVs. Beyond this proof-of-principle demonstration, early functional architectures are realized. In particular, a transmembrane structure acting as a pore is 3D printed, thereby allowing for the transport of biological cargo, including DNA, into the synthetic compartment. These experiments show that two-photon 3D laser microprinting can be an important addition to the existing toolbox of synthetic biology
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|a Journal Article
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|a 3D laser printing
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|a PEGDA hydrogel
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4 |
|a additive manufacturing
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|a bottom-up synthetic biology
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| 650 |
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4 |
|a direct laser writing
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| 650 |
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4 |
|a giant unilamellar lipid vesicles
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| 650 |
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4 |
|a transmembrane pores
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| 650 |
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|a Unilamellar Liposomes
|2 NLM
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| 700 |
1 |
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|a Messer, Tobias
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Jahnke, Kevin
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Hippler, Marc
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Bastmeyer, Martin
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Wegener, Martin
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Göpfrich, Kerstin
|e verfasserin
|4 aut
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| 773 |
0 |
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|i Enthalten in
|t Advanced materials (Deerfield Beach, Fla.)
|d 1998
|g 34(2022), 6 vom: 01. Feb., Seite e2106709
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|g volume:34
|g year:2022
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|g pages:e2106709
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|u http://dx.doi.org/10.1002/adma.202106709
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