Improvement of diagnostic markers for resistance to Globodera pallida and application for selection of resistant germplasms in potato breeding

Copyright © 2021 by JAPANESE SOCIETY OF BREEDING.

Détails bibliographiques
Publié dans:Breeding science. - 1998. - 71(2021), 3 vom: 05. Juni, Seite 354-364
Auteur principal: Asano, Kenji (Auteur)
Autres auteurs: Shimosaka, Etsuo, Yamashita, Yoko, Narabu, Takashi, Aiba, Satoshi, Sakata, Itaru, Akai, Kotaro, Okamoto, Satoshi, Tamiya, Seiji
Format: Article en ligne
Langue:English
Publié: 2021
Accès à la collection:Breeding science
Sujets:Journal Article DNA marker Globodera pallida germplasms marker assisted selection (MAS) potato resistance
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520 |a Occurrence of pale potato cyst nematode, Globodera pallida (Stone) Behrens, was first recorded in Japan in 2015. Among several control measures, cultivation of resistant potato (Solanum tuberosum L.) varieties is the most effective in cost and environmental impact. As no G. pallida-resistant varieties have yet been developed in Japan, great emphasis is being placed on screening of germplasm possessing the resistance and development of the resistant varieties. In this study, we first improved previously reported DNA markers linked to the G. pallida resistance loci (GpaIVs adg and Gpa5) and then used these to screen more than 1,000 germplasms to select several candidate germplasms with resistance. We performed inoculation testing on the selected candidates and identified several resistant germplasms to the Japanese G. pallida population. Furthermore, we developed a simultaneous detection method combining three DNA markers linked to G. pallida and Globodera rostochiensis (Wollenweber) Behrens resistance loci. We validated the ability of C237-I marker to select resistant allele of GpaIVs adg and predict the presence of resistance in a Japanese breeding population. Resistant germplasms identified in this study could potentially be used to develop G. pallida-resistant varieties. The marker evaluation methods developed in this study will contribute to the efficient development of resistant varieties 
650 4 |a Journal Article 
650 4 |a DNA marker 
650 4 |a Globodera pallida 
650 4 |a germplasms 
650 4 |a marker assisted selection (MAS) 
650 4 |a potato 
650 4 |a resistance 
700 1 |a Shimosaka, Etsuo  |e verfasserin  |4 aut 
700 1 |a Yamashita, Yoko  |e verfasserin  |4 aut 
700 1 |a Narabu, Takashi  |e verfasserin  |4 aut 
700 1 |a Aiba, Satoshi  |e verfasserin  |4 aut 
700 1 |a Sakata, Itaru  |e verfasserin  |4 aut 
700 1 |a Akai, Kotaro  |e verfasserin  |4 aut 
700 1 |a Okamoto, Satoshi  |e verfasserin  |4 aut 
700 1 |a Tamiya, Seiji  |e verfasserin  |4 aut 
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773 1 8 |g volume:71  |g year:2021  |g number:3  |g day:05  |g month:06  |g pages:354-364 
856 4 0 |u http://dx.doi.org/10.1270/jsbbs.20134  |3 Volltext 
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