Switch from symplasmic to aspoplasmic phloem unloading in Xanthoceras sorbifolia fruit and sucrose influx XsSWEET10 as a key candidate for Sugar transport
Copyright © 2021 Elsevier B.V. All rights reserved.
| Publié dans: | Plant science : an international journal of experimental plant biology. - 1985. - 313(2021) vom: 01. Dez., Seite 111089 |
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| Auteur principal: | |
| Autres auteurs: | , , , , |
| Format: | Article en ligne |
| Langue: | English |
| Publié: |
2021
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| Accès à la collection: | Plant science : an international journal of experimental plant biology |
| Sujets: | Journal Article Carboxyfluorescein imaging Phloem unloading Sugar metabolism Sugar transporter Xanthoceras sorbifolia Sucrose 57-50-1 |
| Résumé: | Copyright © 2021 Elsevier B.V. All rights reserved. The process of phloem unloading and post-unloading transport of photoassimilate is critical to crop output. Xanthoceras sorbifolia is a woody oil species with great biomass energy prospects in China; however, underproduction of seeds seriously restricts its development. Here, our cytological studies by ultrastructural observation revealed that the sieve element-companion cell complex in carpellary bundle was symplasmically interconnected with surrounding parenchyma cells at the early and late fruit developmental stages, whereas it was symplasmically isolated at middle stage. Consistently, real-time imaging showed that fluorescent tracer 6(5)carboxyfluorescein was confined to phloem strands at middle stage but released into surrounding parenchymal cells at early and late stages. Enzymatic assay showed that sucrose synthase act as the key enzyme catalyzing the progress of Suc degradation post-unloading pathway whether in pericarp or in seed, while vacuolar acid invertase and neutral invertase play compensation roles in sucrose decomposition. Sugar transporter XsSWEET10 had a high expression profile in fruit, especially at middle stage. XsSWEET10 is a plasma membrane-localized protein and heterologous expression in SUC2-deficient yeast strain SUSY7/ura3 confirmed its ability to uptake sucrose. These findings approved the transition from symplasmic to apoplasmic phloem unloading in Xanthoceras sorbifolia fruit and XsSWEET10 as a key candidate in sugar transport |
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| Description: | Date Completed 25.11.2021 Date Revised 25.11.2021 published: Print-Electronic Citation Status MEDLINE |
| ISSN: | 1873-2259 |
| DOI: | 10.1016/j.plantsci.2021.111089 |