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20231225220754.0 |
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231225s2021 xx |||||o 00| ||eng c |
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|a 10.1016/j.plantsci.2021.111083
|2 doi
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2 |
|a pubmed24n1110.xml
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|a (DE-627)NLM333017617
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|a (NLM)34763868
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|a (PII)S0168-9452(21)00279-X
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|a DE-627
|b ger
|c DE-627
|e rakwb
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| 041 |
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|a eng
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| 100 |
1 |
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|a Cerezo, Sergio
|e verfasserin
|4 aut
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| 245 |
1 |
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|a Modification of 13-hydroperoxide lyase expression in olive affects plant growth and results in altered volatile profile
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| 264 |
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|c 2021
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| 336 |
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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| 338 |
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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| 500 |
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|a Date Completed 25.11.2021
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|a Date Revised 25.11.2021
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a Copyright © 2021 The Authors. Published by Elsevier B.V. All rights reserved.
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|a The C6 aldehydes, alcohols, and the corresponding esters are the most important compounds of virgin olive oil aroma. These C6 volatile compounds are synthesized via the 13-hydroperoxide lyase (13-HPL) branch of the lipoxygenase pathway. In this investigation, a functional analysis of the olive (Olea europaea L.) 13-HPL gene by its overexpression and silencing in olive transgenic lines was carried out. With this aim, sense and RNAi constructs of the olive 13-HPL gene were generated and used for the transformation of embryogenic olive cultures. Leaves from overexpressing lines showed a slight increase in 13-HPL gene expression, whereas RNAi lines exhibited a strong decrease in their transcript levels. Quantification of 13-HPL activity in two overexpressing and two RNAi lines showed a positive correlation with levels of transcripts. Interestingly, RNAi lines showed a high decrease in the content of C6 volatiles linked to a strong increase of C5 volatile compounds, altering the volatile profile in the leaves. In addition, the silencing of the 13-HPL gene severely affected plant growth and development. This investigation demonstrates the role of the 13-HPL gene in the biogenesis of olive volatile compounds and constitutes a functional genomics study in olive related to virgin olive oil quality
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|a Journal Article
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|a Gene silencing
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|a HPL
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| 650 |
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|a Olea europaea
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| 650 |
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|a Overexpression
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4 |
|a Transgenic plants
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| 650 |
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4 |
|a Volatiles
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| 650 |
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|a Oils, Volatile
|2 NLM
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| 650 |
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|a Olive Oil
|2 NLM
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| 650 |
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|a Lipoxygenase
|2 NLM
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| 650 |
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7 |
|a EC 1.13.11.12
|2 NLM
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| 700 |
1 |
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|a Hernández, M Luisa
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Palomo-Ríos, Elena
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Gouffi, Naima
|e verfasserin
|4 aut
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| 700 |
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|a García-Vico, Lourdes
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Sicardo, M Dolores
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Sanz, Carlos
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Mercado, José A
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Pliego-Alfaro, Fernando
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Martínez-Rivas, José M
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Plant science : an international journal of experimental plant biology
|d 1985
|g 313(2021) vom: 01. Dez., Seite 111083
|w (DE-627)NLM098174193
|x 1873-2259
|7 nnns
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| 773 |
1 |
8 |
|g volume:313
|g year:2021
|g day:01
|g month:12
|g pages:111083
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| 856 |
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|u http://dx.doi.org/10.1016/j.plantsci.2021.111083
|3 Volltext
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|d 313
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|b 01
|c 12
|h 111083
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