|
|
|
|
| LEADER |
01000naa a22002652 4500 |
| 001 |
NLM333017536 |
| 003 |
DE-627 |
| 005 |
20231225220754.0 |
| 007 |
cr uuu---uuuuu |
| 008 |
231225s2021 xx |||||o 00| ||eng c |
| 024 |
7 |
|
|a 10.1016/j.plantsci.2021.111069
|2 doi
|
| 028 |
5 |
2 |
|a pubmed24n1110.xml
|
| 035 |
|
|
|a (DE-627)NLM333017536
|
| 035 |
|
|
|a (NLM)34763860
|
| 035 |
|
|
|a (PII)S0168-9452(21)00265-X
|
| 040 |
|
|
|a DE-627
|b ger
|c DE-627
|e rakwb
|
| 041 |
|
|
|a eng
|
| 100 |
1 |
|
|a Le, Duc Duy
|e verfasserin
|4 aut
|
| 245 |
1 |
0 |
|a Identification of three groups of ginsenoside biosynthetic UDP-glycosyltransferases from Gynostemma pentaphyllum
|
| 264 |
|
1 |
|c 2021
|
| 336 |
|
|
|a Text
|b txt
|2 rdacontent
|
| 337 |
|
|
|a ƒaComputermedien
|b c
|2 rdamedia
|
| 338 |
|
|
|a ƒa Online-Ressource
|b cr
|2 rdacarrier
|
| 500 |
|
|
|a Date Completed 25.11.2021
|
| 500 |
|
|
|a Date Revised 25.11.2021
|
| 500 |
|
|
|a published: Print-Electronic
|
| 500 |
|
|
|a Citation Status MEDLINE
|
| 520 |
|
|
|a Copyright © 2021 Elsevier B.V. All rights reserved.
|
| 520 |
|
|
|a Ginsenosides are glycosylated dammarene-type triterpenes that have been identified in distantly related Panax ginseng and Gynostemma pentaphyllum. The phylogenetic relatedness of the ginsenoside biosynthetic genes in the two species was previously unknown. The final steps of ginsenoside biosynthesis are the glycosylations of hydroxylated triterpenes, protopanaxadiol (PPD) and protopanaxatriol (PPT), and their glycosylated forms by UDP-glycosyltransferases (UGTs). Ginsenoside biosynthetic UGTs have been identified in Panax but not in Gynostemma. Through a biochemical screening of Gynostemma UGTs (GpUGTs), we herein identified three groups of ginsenoside biosynthetic GpUGTs. These groups comprise: two GpUGTs that belong to the UGT71 family and glucosylate the C20-OH positions of PPD- and PPT-type ginsenosides; one GpUGT that belongs to the UGT74 family and glucosylates the C3-OH position of PPD-type ginsenosides; and two GpUGTs that belong to the UGT94 family and add a glucose to the C3-O-glucosides of PPD-type ginsenosides. These GpUGTs belong to the same UGT families as the ginsenoside biosynthetic Panax UGTs (PgUGTs). However, GpUGTs and PgUGTs belong to different subfamilies. Furthermore, cucumber UGTs orthologous to GpUGTs do not glucosylate ginsenosides. These results collectively suggest that, during evolution, P. ginseng and G. pentaphyllum independently opted to use the same UGT families to synthesize ginsenosides
|
| 650 |
|
4 |
|a Journal Article
|
| 650 |
|
4 |
|a Ginseng
|
| 650 |
|
4 |
|a Ginsenoside
|
| 650 |
|
4 |
|a Gynostemma
|
| 650 |
|
4 |
|a Gypenoside
|
| 650 |
|
4 |
|a Panax
|
| 650 |
|
4 |
|a UDP-glycosyltransferase
|
| 650 |
|
7 |
|a Ginsenosides
|2 NLM
|
| 650 |
|
7 |
|a Glycosyltransferases
|2 NLM
|
| 650 |
|
7 |
|a EC 2.4.-
|2 NLM
|
| 700 |
1 |
|
|a Kim, Woohyun
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Lim, Soohwan
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Kim, Sun Chang
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Choi, Giltsu
|e verfasserin
|4 aut
|
| 773 |
0 |
8 |
|i Enthalten in
|t Plant science : an international journal of experimental plant biology
|d 1985
|g 313(2021) vom: 01. Dez., Seite 111069
|w (DE-627)NLM098174193
|x 1873-2259
|7 nnns
|
| 773 |
1 |
8 |
|g volume:313
|g year:2021
|g day:01
|g month:12
|g pages:111069
|
| 856 |
4 |
0 |
|u http://dx.doi.org/10.1016/j.plantsci.2021.111069
|3 Volltext
|
| 912 |
|
|
|a GBV_USEFLAG_A
|
| 912 |
|
|
|a SYSFLAG_A
|
| 912 |
|
|
|a GBV_NLM
|
| 912 |
|
|
|a GBV_ILN_350
|
| 951 |
|
|
|a AR
|
| 952 |
|
|
|d 313
|j 2021
|b 01
|c 12
|h 111069
|