Functional characterization of (S)-N-methylcoclaurine 3'-hydroxylase (NMCH) involved in the biosynthesis of benzylisoquinoline alkaloids in Corydalis yanhusuo

Functional characterization of (S)-N-methylcoclaurine 3'-hydroxylase (NMCH) involved in the biosynthesis of benzylisoquinoline alkaloids in Corydalis yanhusuo

Copyright © 2021 Elsevier Masson SAS. All rights reserved.

Bibliographische Detailangaben
Veröffentlicht in:Plant physiology and biochemistry : PPB. - 1991. - 168(2021) vom: 15. Nov., Seite 507-515
1. Verfasser: Liu, Xiuyu (VerfasserIn)
Weitere Verfasser: Bu, Junling, Ma, Ying, Chen, Yun, Li, Qishuang, Jiao, Xiang, Hu, Zhimin, Cui, Guanghong, Tang, Jinfu, Guo, Juan, Huang, Luqi
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2021
Zugriff auf das übergeordnete Werk:Plant physiology and biochemistry : PPB
Schlagworte:Journal Article (S)–N-Methylcoclaurine 3′-hydroxylase Corydalis yanhusuo Synthetic biology Alkaloids Benzylisoquinolines Plant Proteins Cytochrome P-450 Enzyme System 9035-51-2 N-methylcoclaurine 3'-hydroxylase CYP80B EC 1.14.14.102
Beschreibung
Zusammenfassung:Copyright © 2021 Elsevier Masson SAS. All rights reserved.
Benzylisoquinoline alkaloids (BIAs) are compounds naturally found in plants and can have significant value in clinical settings. Metabolic engineering and synthetic biology are both promising approaches for the heterologous acquisition of benzylisoquinoline alkaloids. (S)-N-methylcoclaurine 3'-hydroxylase (NMCH), a member of the CYP80 family of CYP450, is the penultimate catalytic enzyme that forms the central branch-point intermediate (S)-reticuline and plays a key role in the biosynthesis of BIAs. In this study, an NMCH gene was cloned from Corydalis yanhusuo, while in vitro reactions demonstrated that CyNMCH can catalyze (S)-N-methylcoclaurine to produce (S)-3'-hydroxy-N-methylcoclaurine. The Km and Kcat of CyNMCH were estimated and compared with those identified in Eschscholzia californica and Coptis japonica. This newly discovered CyNMCH will provide alternative genetic resources for the synthetic biological production of benzylisoquinoline alkaloids and provides a foundation to help analyze the biosynthetic pathway of BIAs biosynthesis in C. yanhusuo
Beschreibung:Date Completed 10.12.2021
Date Revised 14.12.2021
published: Print-Electronic
Citation Status MEDLINE
ISSN:1873-2690
DOI:10.1016/j.plaphy.2021.09.042