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231225s2022 xx |||||o 00| ||eng c |
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|a 10.1002/adma.202106633
|2 doi
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|a pubmed24n1108.xml
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|a (DE-627)NLM332485684
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|a (NLM)34710248
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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1 |
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|a Zhao, Ziliang
|e verfasserin
|4 aut
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|a Super-Resolution Imaging of Highly Curved Membrane Structures in Giant Vesicles Encapsulating Molecular Condensates
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|c 2022
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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|a Date Completed 31.03.2022
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|a Date Revised 01.04.2022
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a © 2021 The Authors. Advanced Materials published by Wiley-VCH GmbH.
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|a Molecular crowding is an inherent feature of cell interiors. Synthetic cells as provided by giant unilamellar vesicles (GUVs) encapsulating macromolecules (poly(ethylene glycol) and dextran) represent an excellent mimetic system to study membrane transformations associated with molecular crowding and protein condensation. Similarly to cells, such GUVs exhibit highly curved structures like nanotubes. Upon liquid-liquid phase separation their membrane deforms into apparent kinks at the contact line of the interface between the two aqueous phases. These structures, nanotubes, and kinks, have dimensions below optical resolution. Here, these are studied with super-resolution stimulated emission depletion (STED) microscopy facilitated by immobilization in a microfluidic device. The cylindrical nature of the nanotubes based on the superior resolution of STED and automated data analysis is demonstrated. The deduced membrane spontaneous curvature is in excellent agreement with theoretical predictions. Furthermore, the membrane kink-like structure is resolved as a smoothly curved membrane demonstrating the existence of the intrinsic contact angle, which describes the wettability contrast of the encapsulated phases to the membrane. Resolving these highly curved membrane structures with STED imaging provides important insights in the membrane properties and interactions underlying cellular activities
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|a Journal Article
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|a aqueous two-phase systems (ATPSs)
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4 |
|a giant unilamellar vesicles (GUVs)
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4 |
|a membrane nanotubes
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4 |
|a microfluidics
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4 |
|a polymers
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4 |
|a spontaneous curvature
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4 |
|a stimulated emission depletion (STED)
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| 650 |
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|a Proteins
|2 NLM
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| 650 |
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|a Unilamellar Liposomes
|2 NLM
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| 700 |
1 |
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|a Roy, Debjit
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Steinkühler, Jan
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Robinson, Tom
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Lipowsky, Reinhard
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Dimova, Rumiana
|e verfasserin
|4 aut
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| 773 |
0 |
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|i Enthalten in
|t Advanced materials (Deerfield Beach, Fla.)
|d 1998
|g 34(2022), 4 vom: 01. Jan., Seite e2106633
|w (DE-627)NLM098206397
|x 1521-4095
|7 nnns
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| 773 |
1 |
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|g volume:34
|g year:2022
|g number:4
|g day:01
|g month:01
|g pages:e2106633
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|u http://dx.doi.org/10.1002/adma.202106633
|3 Volltext
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