Mutually opposing activity of PIN7 splicing isoforms is required for auxin-mediated tropic responses in Arabidopsis thaliana

Mutually opposing activity of PIN7 splicing isoforms is required for auxin-mediated tropic responses in Arabidopsis thaliana

© 2021 The Authors. New Phytologist © 2021 New Phytologist Foundation.

Bibliographische Detailangaben
Veröffentlicht in:The New phytologist. - 1979. - 233(2022), 1 vom: 12. Jan., Seite 329-343
1. Verfasser: Kashkan, Ivan (VerfasserIn)
Weitere Verfasser: Hrtyan, Mónika, Retzer, Katarzyna, Humpolíčková, Jana, Jayasree, Aswathy, Filepová, Roberta, Vondráková, Zuzana, Simon, Sibu, Rombaut, Debbie, Jacobs, Thomas B, Frilander, Mikko J, Hejátko, Jan, Friml, Jiří, Petrášek, Jan, Růžička, Kamil
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2022
Zugriff auf das übergeordnete Werk:The New phytologist
Schlagworte:Journal Article Research Support, Non-U.S. Gov't Arabidopsis thaliana FRAP PINs RNA processing alternative splicing auxin auxin transport plant development mehr... Arabidopsis Proteins Indoleacetic Acids Protein Isoforms
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520 |a Advanced transcriptome sequencing has revealed that the majority of eukaryotic genes undergo alternative splicing (AS). Nonetheless, little effort has been dedicated to investigating the functional relevance of particular splicing events, even those in the key developmental and hormonal regulators. Combining approaches of genetics, biochemistry and advanced confocal microscopy, we describe the impact of alternative splicing on the PIN7 gene in the model plant Arabidopsis thaliana. PIN7 encodes a polarly localized transporter for the phytohormone auxin and produces two evolutionarily conserved transcripts, PIN7a and PIN7b. PIN7a and PIN7b, differing in a four amino acid stretch, exhibit almost identical expression patterns and subcellular localization. We reveal that they are closely associated and mutually influence each other's mobility within the plasma membrane. Phenotypic complementation tests indicate that the functional contribution of PIN7b per se is minor, but it markedly reduces the prominent PIN7a activity, which is required for correct seedling apical hook formation and auxin-mediated tropic responses. Our results establish alternative splicing of the PIN family as a conserved, functionally relevant mechanism, revealing an additional regulatory level of auxin-mediated plant development 
650 4 |a Journal Article 
650 4 |a Research Support, Non-U.S. Gov't 
650 4 |a Arabidopsis thaliana 
650 4 |a FRAP 
650 4 |a PINs 
650 4 |a RNA processing 
650 4 |a alternative splicing 
650 4 |a auxin 
650 4 |a auxin transport 
650 4 |a plant development 
650 7 |a Arabidopsis Proteins  |2 NLM 
650 7 |a Indoleacetic Acids  |2 NLM 
650 7 |a Protein Isoforms  |2 NLM 
700 1 |a Hrtyan, Mónika  |e verfasserin  |4 aut 
700 1 |a Retzer, Katarzyna  |e verfasserin  |4 aut 
700 1 |a Humpolíčková, Jana  |e verfasserin  |4 aut 
700 1 |a Jayasree, Aswathy  |e verfasserin  |4 aut 
700 1 |a Filepová, Roberta  |e verfasserin  |4 aut 
700 1 |a Vondráková, Zuzana  |e verfasserin  |4 aut 
700 1 |a Simon, Sibu  |e verfasserin  |4 aut 
700 1 |a Rombaut, Debbie  |e verfasserin  |4 aut 
700 1 |a Jacobs, Thomas B  |e verfasserin  |4 aut 
700 1 |a Frilander, Mikko J  |e verfasserin  |4 aut 
700 1 |a Hejátko, Jan  |e verfasserin  |4 aut 
700 1 |a Friml, Jiří  |e verfasserin  |4 aut 
700 1 |a Petrášek, Jan  |e verfasserin  |4 aut 
700 1 |a Růžička, Kamil  |e verfasserin  |4 aut 
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773 1 8 |g volume:233  |g year:2022  |g number:1  |g day:12  |g month:01  |g pages:329-343 
856 4 0 |u http://dx.doi.org/10.1111/nph.17792  |3 Volltext 
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