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231225s2021 xx |||||o 00| ||eng c |
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|a 10.2144/btn-2021-0057
|2 doi
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|e rakwb
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|a eng
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| 100 |
1 |
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|a Echeverría-Beirute, Fabián
|e verfasserin
|4 aut
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| 245 |
1 |
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|a eDNA extraction protocol for metagenomic studies in tropical soils
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|c 2021
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| 336 |
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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| 338 |
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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| 500 |
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|a Date Completed 16.03.2022
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|a Date Revised 16.03.2022
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a The lack of knowledge about biological communities residing in soils, especially those in tropical regions, represents a constraint to management practices to take advantage of the ecological services provided by soil microbiota to agroecosystems. One of the complexities derived from describing biological diversity in such tropical conditions comes from the methods used to isolate microorganisms without altering the composition of the sample. The goal of this study was to establish a protocol for adequate soil sampling and environmental DNA extraction from a tropical region in Costa Rica. We present an up-to-date protocol optimized for tropical soils which improves sevenfold the amount of DNA extracted without significantly affecting the 260/280 and 260/230 ratios compared with commercially available kits and standard protocols
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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| 650 |
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|a communities
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| 650 |
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|a conservation
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| 650 |
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|a metagenomics
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| 650 |
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4 |
|a pineapple
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| 650 |
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|a protocol
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| 650 |
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|a storage
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| 650 |
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|a tropics
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| 650 |
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|a DNA, Environmental
|2 NLM
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| 650 |
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|a Soil
|2 NLM
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| 700 |
1 |
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|a Varela-Benavides, Ingrid
|e verfasserin
|4 aut
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| 700 |
1 |
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|a P Jiménez-Madrigal, Jose
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Carvajal-Chacon, Milagro
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Guzmán-Hernández, Tomás
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t BioTechniques
|d 1988
|g 71(2021), 6 vom: 16. Dez., Seite 580-586
|w (DE-627)NLM012627046
|x 1940-9818
|7 nnns
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| 773 |
1 |
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|g volume:71
|g year:2021
|g number:6
|g day:16
|g month:12
|g pages:580-586
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|u http://dx.doi.org/10.2144/btn-2021-0057
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