Studies on the tissue specific nature and stress inducible activation of the CHI-1 gene from banana

Copyright © 2021 Elsevier Masson SAS. All rights reserved.

Bibliographische Detailangaben
Veröffentlicht in:Plant physiology and biochemistry : PPB. - 1991. - 168(2021) vom: 01. Nov., Seite 62-69
1. Verfasser: Negi, Sanjana (VerfasserIn)
Weitere Verfasser: Madari, Steffi, Tak, Himanshu, Bhakta, Subham, Ganapathi, T R
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2021
Zugriff auf das übergeordnete Werk:Plant physiology and biochemistry : PPB
Schlagworte:Journal Article Banana CHI-1 GUS Promoter Tobacco Transgenic Abscisic Acid 72S9A8J5GW
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520 |a In the present study, the 5'-regulatory region of chalcone isomerase gene (MusaCHI-1) of banana was functionally analysed for its tissue specific, stress mediated and strong guard cell preferred activity. Expression of MusaCHI-1 was altered in leaves of banana plants exposed to various stress conditions and signalling molecules. Transgenic lines of tobacco harbouring PMusaCHI-1-GUS displays prominent GUS staining in vascular region and guard cells of leaves which corroborates with array of Dof1 binding cis-elements in PMusaCHI-1 region. Multiple cis-elements associated with various stress conditions were detected in PMusaCHI-1 which directly correlates with alteration of MusaCHI-1 transcript level in banana exposed to stress conditions. GUS staining of transgenic tobacco plants harbouring PMusaCHI-1-GUS and exposed to drought, salinity, and applications of methyl jasmonate and abscisic acid indicated activation of PMusaCHI-1 under these conditions while exposure of salicylic acid strongly suppresses GUS expression from PMusaCHI-1 
650 4 |a Journal Article 
650 4 |a Banana 
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650 4 |a GUS 
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700 1 |a Madari, Steffi  |e verfasserin  |4 aut 
700 1 |a Tak, Himanshu  |e verfasserin  |4 aut 
700 1 |a Bhakta, Subham  |e verfasserin  |4 aut 
700 1 |a Ganapathi, T R  |e verfasserin  |4 aut 
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