Overexpression of Ginkgo BBX25 enhances salt tolerance in Transgenic Populus

Copyright © 2021 Elsevier Masson SAS. All rights reserved.

Bibliographische Detailangaben
Veröffentlicht in:Plant physiology and biochemistry : PPB. - 1991. - 167(2021) vom: 20. Okt., Seite 946-954
1. Verfasser: Huang, Shujing (VerfasserIn)
Weitere Verfasser: Chen, Caihui, Xu, Mengxuan, Wang, Guibin, Xu, Li-An, Wu, Yaqiong
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2021
Zugriff auf das übergeordnete Werk:Plant physiology and biochemistry : PPB
Schlagworte:Journal Article Abiotic stress BBX Ginkgo Overexpression Transgenic Populus Plant Proteins
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520 |a B-box (BBX) genes play important roles in plant growth, light morphogenesis, and environmental stress responses. Ginkgo (Ginkgo biloba L.) is known as a living fossil species that has a strong ability to adapt to environmental changes and tolerate harsh conditions. In this study, we chose this species to investigate the function of the GbBBX25 gene. We isolated the BBX gene from ginkgo and named it GbBBX25; this gene consists of an 819 bp open reading frame (ORF) that encodes 273 amino acids with two B-box domains but no CCT domain. GbBBX25 was localized in only the nucleus. The expression of GbBBX25 transcripts was observed in the leaves and was significantly enhanced under salt stress conditions. To further verify its function, we overexpressed the GbBBX25 gene in Populus davidiana × Populus bolleana and found that the transgenic Populus had greater soluble sugar levels and higher peroxidase (POD) activity in response to salt stress than nontransgenic (NT) Populus. Five genes related to salt stress were induced in transgenic plants with significantly higher expression levels than those in NT plants. This finding suggests that GbBBX25 improves the salt adaptation abilities of transgenic Populus and provides a scientific basis for related research 
650 4 |a Journal Article 
650 4 |a Abiotic stress 
650 4 |a BBX 
650 4 |a Ginkgo 
650 4 |a Overexpression 
650 4 |a Transgenic Populus 
650 7 |a Plant Proteins  |2 NLM 
700 1 |a Chen, Caihui  |e verfasserin  |4 aut 
700 1 |a Xu, Mengxuan  |e verfasserin  |4 aut 
700 1 |a Wang, Guibin  |e verfasserin  |4 aut 
700 1 |a Xu, Li-An  |e verfasserin  |4 aut 
700 1 |a Wu, Yaqiong  |e verfasserin  |4 aut 
773 0 8 |i Enthalten in  |t Plant physiology and biochemistry : PPB  |d 1991  |g 167(2021) vom: 20. Okt., Seite 946-954  |w (DE-627)NLM098178261  |x 1873-2690  |7 nnns 
773 1 8 |g volume:167  |g year:2021  |g day:20  |g month:10  |g pages:946-954 
856 4 0 |u http://dx.doi.org/10.1016/j.plaphy.2021.09.021  |3 Volltext 
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