|
|
|
|
| LEADER |
01000caa a22002652 4500 |
| 001 |
NLM330963600 |
| 003 |
DE-627 |
| 005 |
20241013231824.0 |
| 007 |
cr uuu---uuuuu |
| 008 |
231225s2021 xx |||||o 00| ||eng c |
| 024 |
7 |
|
|a 10.1002/adma.202104581
|2 doi
|
| 028 |
5 |
2 |
|a pubmed24n1566.xml
|
| 035 |
|
|
|a (DE-627)NLM330963600
|
| 035 |
|
|
|a (NLM)34554608
|
| 040 |
|
|
|a DE-627
|b ger
|c DE-627
|e rakwb
|
| 041 |
|
|
|a eng
|
| 100 |
1 |
|
|a Giaveri, Simone
|e verfasserin
|4 aut
|
| 245 |
1 |
0 |
|a Nature-Inspired Circular-Economy Recycling for Proteins
|b Proof of Concept
|
| 264 |
|
1 |
|c 2021
|
| 336 |
|
|
|a Text
|b txt
|2 rdacontent
|
| 337 |
|
|
|a ƒaComputermedien
|b c
|2 rdamedia
|
| 338 |
|
|
|a ƒa Online-Ressource
|b cr
|2 rdacarrier
|
| 500 |
|
|
|a Date Completed 24.07.2024
|
| 500 |
|
|
|a Date Revised 13.10.2024
|
| 500 |
|
|
|a published: Print-Electronic
|
| 500 |
|
|
|a Citation Status MEDLINE
|
| 520 |
|
|
|a © 2021 The Authors. Advanced Materials published by Wiley-VCH GmbH.
|
| 520 |
|
|
|a The billion tons of synthetic-polymer-based materials (i.e. plastics) produced yearly are a great challenge for humanity. Nature produces even more natural polymers, yet they are sustainable. Proteins are sequence-defined natural polymers that are constantly recycled when living systems feed. Digestion is the protein depolymerization into amino acids (the monomers) followed by their re-assembly into new proteins of arbitrarily different sequence and function. This breaks a common recycling paradigm where a material is recycled into itself. Organisms feed off of random protein mixtures that are "recycled" into new proteins whose identity depends on the cell's specific needs. In this study, mixtures of several peptides and/or proteins are depolymerized into their amino acid constituents, and these amino acids are used to synthesize new fluorescent, and bioactive proteins extracellularly by using an amino-acid-free, cell-free transcription-translation (TX-TL) system. Specifically, three peptides (magainin II, glucagon, and somatostatin 28) are digested using thermolysin first and then using leucine aminopeptidase. The amino acids so produced are added to a commercial TX-TL system to produce fluorescent proteins. Furthermore, proteins with high relevance in materials engineering (β-lactoglobulin films, used for water filtration, or silk fibroin solutions) are successfully recycled into biotechnologically relevant proteins (fluorescent proteins, catechol 2,3-dioxygenase)
|
| 650 |
|
4 |
|a Journal Article
|
| 650 |
|
4 |
|a protein-based materials
|
| 650 |
|
4 |
|a recycling
|
| 650 |
|
4 |
|a sequence-defined polymers
|
| 650 |
|
4 |
|a sustainability
|
| 650 |
|
7 |
|a Proteins
|2 NLM
|
| 650 |
|
7 |
|a Peptides
|2 NLM
|
| 650 |
|
7 |
|a Amino Acids
|2 NLM
|
| 650 |
|
7 |
|a Somatostatin
|2 NLM
|
| 650 |
|
7 |
|a 51110-01-1
|2 NLM
|
| 650 |
|
7 |
|a Lactoglobulins
|2 NLM
|
| 650 |
|
7 |
|a Leucyl Aminopeptidase
|2 NLM
|
| 650 |
|
7 |
|a EC 3.4.11.1
|2 NLM
|
| 700 |
1 |
|
|a Schmitt, Adeline Marie
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Roset Julià, Laura
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Scamarcio, Vincenzo
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Murello, Anna
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Cheng, Shiyu
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Menin, Laure
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Ortiz, Daniel
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Patiny, Luc
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Bolisetty, Sreenath
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Mezzenga, Raffaele
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Maerkl, Sebastian Josef
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Stellacci, Francesco
|e verfasserin
|4 aut
|
| 773 |
0 |
8 |
|i Enthalten in
|t Advanced materials (Deerfield Beach, Fla.)
|d 1998
|g 33(2021), 44 vom: 30. Nov., Seite e2104581
|w (DE-627)NLM098206397
|x 1521-4095
|7 nnns
|
| 773 |
1 |
8 |
|g volume:33
|g year:2021
|g number:44
|g day:30
|g month:11
|g pages:e2104581
|
| 856 |
4 |
0 |
|u http://dx.doi.org/10.1002/adma.202104581
|3 Volltext
|
| 912 |
|
|
|a GBV_USEFLAG_A
|
| 912 |
|
|
|a SYSFLAG_A
|
| 912 |
|
|
|a GBV_NLM
|
| 912 |
|
|
|a GBV_ILN_350
|
| 951 |
|
|
|a AR
|
| 952 |
|
|
|d 33
|j 2021
|e 44
|b 30
|c 11
|h e2104581
|