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231225s2021 xx |||||o 00| ||eng c |
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|a 10.1002/adma.202104615
|2 doi
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|a pubmed24n1103.xml
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|a (DE-627)NLM330951769
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|a (NLM)34553420
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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| 100 |
1 |
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|a Jia, Hui
|e verfasserin
|4 aut
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| 245 |
1 |
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|a AIEgen-Based Lifetime-Probes for Precise Furin Quantification and Identification of Cell Subtypes
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|c 2021
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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|a Date Completed 23.02.2022
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|a Date Revised 23.02.2022
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a © 2021 Wiley-VCH GmbH.
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|a Biochemical sensing probes based on aggregation-induced-emission luminogens (AIEgens) are widely used in biological imaging and therapy, chemical sensing, and material sciences. However, it is still a great challenge to quantify the targets through fluorescence intensity of AIEgen probes due to their undesirable aggregations. Here, a PyTPA-ZGO probe with three lifetime signals for precise quantification of furin is constructed: the lifetime signal 1 and signal 2 comes from AIEgen PyTPA-P (τPn ) and inorganic nanoparticles Zn2 GeO4 :Mn2+ -NH2 (τZn ), respectively, while the lifetime signal 3 is marked as the composite dual-lifetime signal (CDLSn , C D L S n = τ Z n τ P n ). In contrast, the fluorescence intensity signal of PyTPA-P shows defectively quantitative performance. Furthermore, it is found that the CDLSn exhibits higher significant differences than the two other lifetime signals (τPn and τZn ) thanks to its wide range between the maximum and minimum signal values and small standard deviation. Therefore, CDLSn is further used to accurately identify cell subtypes based on the specific concentration of furin in each subtype. The lifetime criterion can realize precise quantification, and it should be a promising direction of AIEgen-based quantitative analysis in the future
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|a Journal Article
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|a AIEgens
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|a cell subtypes
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|a furin
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|a lifetime
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|a quantification
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|a Fluorescent Dyes
|2 NLM
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|a Oxides
|2 NLM
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|a Peptides
|2 NLM
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|a zinc germanate
|2 NLM
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|a Germanium
|2 NLM
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| 650 |
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|a 00072J7XWS
|2 NLM
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| 650 |
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|a Furin
|2 NLM
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| 650 |
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|a EC 3.4.21.75
|2 NLM
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| 700 |
1 |
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|a Ding, Defang
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Hu, Jingjing
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Dai, Jun
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Yang, Juliang
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Li, Guogang
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Lou, Xiaoding
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Xia, Fan
|e verfasserin
|4 aut
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| 773 |
0 |
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|i Enthalten in
|t Advanced materials (Deerfield Beach, Fla.)
|d 1998
|g 33(2021), 45 vom: 01. Nov., Seite e2104615
|w (DE-627)NLM098206397
|x 1521-4095
|7 nnns
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| 773 |
1 |
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|g volume:33
|g year:2021
|g number:45
|g day:01
|g month:11
|g pages:e2104615
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| 856 |
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|u http://dx.doi.org/10.1002/adma.202104615
|3 Volltext
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