Morphological and molecular characterization of Paratylenchus beltsvillensis n. sp. (Tylenchida Paratylenchidae) from the rhizosphere of pine tree (Pinus virginiana Mill) in Maryland, USA

Morphological and molecular characterization of Paratylenchus beltsvillensis n. sp. (Tylenchida : Paratylenchidae) from the rhizosphere of pine tree (Pinus virginiana Mill) in Maryland, USA

© 2021 Authors.

Bibliographische Detailangaben
Veröffentlicht in:Journal of nematology. - 1969. - 53(2021) vom: 10.
1. Verfasser: Kantor, Mihail R (VerfasserIn)
Weitere Verfasser: Handoo, Zafar A, Subbotin, Sergei A, Bauchan, Gary R, Mowery, Joseph D
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2021
Zugriff auf das übergeordnete Werk:Journal of nematology
Schlagworte:Journal Article D2-D3 of 28S rRNA gene Description ITSrRNA gene Morphology Morphometrics Phylogeny Pin nematode Pinus virginiana Scanning electron microscopy Virginia pine
Beschreibung
Zusammenfassung:© 2021 Authors.
The pin nematode, Paratylechus beltsvillensis n. sp. collected from rhizosphere soil of a Virginia pine tree (Pinus virginiana Mill) growing in Little Paint Branch Park, Beltsville, Prince George's County, Maryland, USA, is described and illustrated along with light and scanning electron photomicrographs. Females, males, and juveniles of this new species were recovered from soil samples using the sugar centrifugal flotation and Baermann funnel extraction methods. Morphologically, females are short, body length ranging from 245 to 267 μm, stylet from 70 to 75 μm long with anchor shaped knobs, vulva located at 70-73% and small vulval flap, spermatheca large, and ovoid filled with sperms. Lateral field with three incisures, of which the outer two are prominent. Tail slender, having a rounded tail terminus. Males without stylet and have a degenerated pharynx, spicules = 17-20 µm and gubernaculum = 5.0-5.5 µm. Both morphological observations and molecular analysis of ITS and partial 28S ribosomal RNA gene sequences indicated that the specimens collected from the soil at Beltsville Park from rhizosphere soil samples from Virginia pine represents a new pin nematode species
Beschreibung:Date Revised 21.09.2021
published: Electronic-eCollection
Citation Status PubMed-not-MEDLINE
ISSN:0022-300X
DOI:10.21307/jofnem-2021-079