|
|
|
|
| LEADER |
01000naa a22002652 4500 |
| 001 |
NLM330758160 |
| 003 |
DE-627 |
| 005 |
20231225211955.0 |
| 007 |
cr uuu---uuuuu |
| 008 |
231225s2022 xx |||||o 00| ||eng c |
| 024 |
7 |
|
|a 10.1111/nph.17739
|2 doi
|
| 028 |
5 |
2 |
|a pubmed24n1102.xml
|
| 035 |
|
|
|a (DE-627)NLM330758160
|
| 035 |
|
|
|a (NLM)34533849
|
| 040 |
|
|
|a DE-627
|b ger
|c DE-627
|e rakwb
|
| 041 |
|
|
|a eng
|
| 100 |
1 |
|
|a Sabatino, Valeria
|e verfasserin
|4 aut
|
| 245 |
1 |
0 |
|a Silencing of a Pseudo-nitzschia arenysensis lipoxygenase transcript leads to reduced oxylipin production and impaired growth
|
| 264 |
|
1 |
|c 2022
|
| 336 |
|
|
|a Text
|b txt
|2 rdacontent
|
| 337 |
|
|
|a ƒaComputermedien
|b c
|2 rdamedia
|
| 338 |
|
|
|a ƒa Online-Ressource
|b cr
|2 rdacarrier
|
| 500 |
|
|
|a Date Completed 24.03.2022
|
| 500 |
|
|
|a Date Revised 24.03.2022
|
| 500 |
|
|
|a published: Print-Electronic
|
| 500 |
|
|
|a Citation Status MEDLINE
|
| 520 |
|
|
|a © 2021 The Authors. New Phytologist © 2021 New Phytologist Foundation.
|
| 520 |
|
|
|a Because of their importance as chemical mediators, the presence of a rich and varied family of lipoxygenase (LOX) products, collectively named oxylipins, has been investigated thoroughly in diatoms, and the involvement of these products in important processes such as bloom regulation has been postulated. Nevertheless, little information is available on the enzymes and pathways operating in these protists. Exploiting transcriptome data, we identified and characterized a LOX gene, PaLOX, in Pseudo-nitzschia arenysensis, a marine diatom known to produce different species of oxylipins by stereo- and regio-selective oxidation of eicosapentaenoic acid (EPA) at C12 and C15. PaLOX RNA interference correlated with a decrease of the lipid-peroxidizing activity and oxylipin synthesis, as well as with a reduction of growth of P. arenysensis. In addition, sequence analysis and structure models of the C-terminal part of the predicted protein closely fitted with the data for established LOXs from other organisms. The presence in the genome of a single LOX gene, whose downregulation impairs both 12- and 15-oxylipins synthesis, together with the in silico 3D protein modelling suggest that PaLOX encodes for a 12/15S-LOX with a dual specificity, and provides additional support to the correlation between cell growth and oxylipin biosynthesis in diatoms
|
| 650 |
|
4 |
|a Journal Article
|
| 650 |
|
4 |
|a Research Support, Non-U.S. Gov't
|
| 650 |
|
4 |
|a Pseudo-nitzschia arenysensis
|
| 650 |
|
4 |
|a RNA-interference
|
| 650 |
|
4 |
|a algal growth
|
| 650 |
|
4 |
|a biosynthesis
|
| 650 |
|
4 |
|a diatoms
|
| 650 |
|
4 |
|a gene function
|
| 650 |
|
7 |
|a Oxylipins
|2 NLM
|
| 650 |
|
7 |
|a Lipoxygenase
|2 NLM
|
| 650 |
|
7 |
|a EC 1.13.11.12
|2 NLM
|
| 700 |
1 |
|
|a Orefice, Ida
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Marotta, Pina
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Ambrosino, Luca
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Chiusano, Maria Luisa
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a d'Ippolito, Giuliana
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Romano, Giovanna
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Fontana, Angelo
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Ferrante, Maria Immacolata
|e verfasserin
|4 aut
|
| 773 |
0 |
8 |
|i Enthalten in
|t The New phytologist
|d 1979
|g 233(2022), 2 vom: 16. Jan., Seite 809-822
|w (DE-627)NLM09818248X
|x 1469-8137
|7 nnns
|
| 773 |
1 |
8 |
|g volume:233
|g year:2022
|g number:2
|g day:16
|g month:01
|g pages:809-822
|
| 856 |
4 |
0 |
|u http://dx.doi.org/10.1111/nph.17739
|3 Volltext
|
| 912 |
|
|
|a GBV_USEFLAG_A
|
| 912 |
|
|
|a SYSFLAG_A
|
| 912 |
|
|
|a GBV_NLM
|
| 912 |
|
|
|a GBV_ILN_350
|
| 951 |
|
|
|a AR
|
| 952 |
|
|
|d 233
|j 2022
|e 2
|b 16
|c 01
|h 809-822
|