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231225s2021 xx |||||o 00| ||eng c |
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|a 10.1111/nph.17737
|2 doi
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|a pubmed25n1102.xml
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|a (DE-627)NLM330672428
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|a (NLM)34525227
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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| 100 |
1 |
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|a Yoda, Akiyoshi
|e verfasserin
|4 aut
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| 245 |
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|a Strigolactone biosynthesis catalyzed by cytochrome P450 and sulfotransferase in sorghum
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|c 2021
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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| 338 |
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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| 500 |
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|a Date Completed 06.01.2022
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|a Date Revised 31.07.2022
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a © 2021 The Authors. New Phytologist © 2021 New Phytologist Foundation.
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|a Root parasitic plants such as Striga, Orobanche, and Phelipanche spp. cause serious damage to crop production world-wide. Deletion of the Low Germination Stimulant 1 (LGS1) gene gives a Striga-resistance trait in sorghum (Sorghum bicolor). The LGS1 gene encodes a sulfotransferase-like protein, but its function has not been elucidated. Since the profile of strigolactones (SLs) that induce seed germination in root parasitic plants is altered in the lgs1 mutant, LGS1 is thought to be an SL biosynthetic enzyme. In order to clarify the enzymatic function of LGS1, we looked for candidate SL substrates that accumulate in the lgs1 mutants and performed in vivo and in vitro metabolism experiments. We found the SL precursor 18-hydroxycarlactonoic acid (18-OH-CLA) is a substrate for LGS1. CYP711A cytochrome P450 enzymes (SbMAX1 proteins) in sorghum produce 18-OH-CLA. When LGS1 and SbMAX1 coding sequences were co-expressed in Nicotiana benthamiana with the upstream SL biosynthesis genes from sorghum, the canonical SLs 5-deoxystrigol and 4-deoxyorobanchol were produced. This finding showed that LGS1 in sorghum uses a sulfo group to catalyze leaving of a hydroxyl group and cyclization of 18-OH-CLA. A similar SL biosynthetic pathway has not been found in other plant species
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a Research Support, U.S. Gov't, Non-P.H.S.
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| 650 |
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|a Sorghum bicolor
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| 650 |
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|a Striga
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|a biosynthesis
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|a cytochrome P450
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|a root parasitic plants
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|a strigolactone
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|a sulfotransferase
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|a GR24 strigolactone
|2 NLM
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| 650 |
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|a Heterocyclic Compounds, 3-Ring
|2 NLM
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| 650 |
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|a Lactones
|2 NLM
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| 650 |
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|a Cytochrome P-450 Enzyme System
|2 NLM
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| 650 |
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|a 9035-51-2
|2 NLM
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| 650 |
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|a Sulfotransferases
|2 NLM
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| 650 |
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|a EC 2.8.2.-
|2 NLM
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| 700 |
1 |
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|a Mori, Narumi
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Akiyama, Kohki
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Kikuchi, Mayu
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Xie, Xiaonan
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Miura, Kenji
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Yoneyama, Kaori
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Sato-Izawa, Kanna
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Yamaguchi, Shinjiro
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Yoneyama, Koichi
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Nelson, David C
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Nomura, Takahito
|e verfasserin
|4 aut
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| 773 |
0 |
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|i Enthalten in
|t The New phytologist
|d 1984
|g 232(2021), 5 vom: 01. Dez., Seite 1999-2010
|w (DE-627)NLM09818248X
|x 1469-8137
|7 nnas
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| 773 |
1 |
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|g volume:232
|g year:2021
|g number:5
|g day:01
|g month:12
|g pages:1999-2010
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| 856 |
4 |
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|u http://dx.doi.org/10.1111/nph.17737
|3 Volltext
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|a GBV_ILN_350
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|a AR
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|d 232
|j 2021
|e 5
|b 01
|c 12
|h 1999-2010
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