Improved SARS-CoV-2 PCR detection and genotyping with double-bubble primers
A new approach for improved RT-PCR is described. It is based on primers designed to form controlled stem-loop and homodimer configurations, hence the name 'double-bubble' primers. The primers contain three main regions for efficient RT-PCR: a 3' short overhang to allow reverse transcr...
| Veröffentlicht in: | BioTechniques. - 1993. - 71(2021), 6 vom: 01. Dez., Seite 587-597 |
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| Format: | Online-Aufsatz |
| Sprache: | English |
| Veröffentlicht: |
2021
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| Zugriff auf das übergeordnete Werk: | BioTechniques |
| Schlagworte: | Journal Article Research Support, Non-U.S. Gov't RT-qPCR SARS-CoV-2 cost-effective PCR double-bubble PCR primers fast PCR hot-start PCR DNA Primers RNA, Viral |
| Online verfügbar |
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