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20240909232014.0 |
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231225s2021 xx |||||o 00| ||eng c |
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|a 10.1016/j.clim.2021.108850
|2 doi
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|a pubmed24n1528.xml
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|a (DE-627)NLM330491628
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|a (NLM)34506944
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|a (PII)S1521-6616(21)00187-X
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|a DE-627
|b ger
|c DE-627
|e rakwb
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| 041 |
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|a eng
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| 100 |
1 |
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|a Mahallawi, Waleed H
|e verfasserin
|4 aut
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| 245 |
1 |
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|a Infection with SARS-CoV-2 primes immunological memory in human nasal-associated lymphoid tissue
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1 |
|c 2021
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| 336 |
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|a Text
|b txt
|2 rdacontent
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| 337 |
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|a ƒaComputermedien
|b c
|2 rdamedia
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| 338 |
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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| 500 |
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|a Date Completed 13.10.2021
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|a Date Revised 08.09.2024
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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| 520 |
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|a Copyright © 2021 Elsevier Inc. All rights reserved.
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|a BACKGROUND: The coronavirus disease 2019 (COVID-19) pandemic, caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection, has resulted in considerable morbidity and mortality in humans. Little is known regarding the development of immunological memory following SARS-CoV-2 infection or whether immunological memory can provide long-lasting protection against reinfection. Urgent need for vaccines is a considerable issue for all governments worldwide
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| 520 |
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|a METHODS: A total of 39 patients were recruited in this study. Tonsillar mononuclear cells (MNCs) were co-cultured in RPMI medium and stimulated with the full-length SARS-CoV-2 spike protein in the presence and absence of a CpG-DNA adjuvant. An enzyme-linked immunosorbent assay (ELISA) was utilised to measure the specific antibody response to the spike protein in the cell culture supernatants
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| 520 |
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|a RESULTS: The SARS-CoV-2 spike protein primed a potent memory B cell-mediated immune response in nasal-associated lymphoid tissue (NALT) from patients previously infected with the virus. Additionally, spike protein combined with the CpG-DNA adjuvant induced a significantly increased level of specific anti-spike protein IgG antibody compared with the spike protein alone (p < 0.0001, n = 24). We also showed a strong positive correlation between the specific anti-spike protein IgG antibody level in a serum samples and that produced by MNCs derived from the same COVID-19-recovered patients following stimulation (r = 0.76, p = 0.0002, n = 24)
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| 520 |
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|a CONCLUSION: Individuals with serological evidence of previous SARS-CoV-2 exposure showed a significant anti-spike protein-specific memory humoral immune response to the viral spike protein upon stimulation. Additionally, our results demonstrated the functional response of NALT-derived MNCs to the viral spike protein. CpG-DNA adjuvant combined with spike protein induced significantly stronger humoral immune responses than the spike protein alone. These data indicate that the S protein antigen combined with CpG-DNA adjuvant could be used as a future vaccine candidate
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| 650 |
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4 |
|a Journal Article
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| 650 |
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4 |
|a CpG-DNA
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| 650 |
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4 |
|a Memory B cells
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| 650 |
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4 |
|a SARS-CoV-2
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| 650 |
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4 |
|a Spike protein
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| 650 |
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7 |
|a Antibodies, Viral
|2 NLM
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| 650 |
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7 |
|a Immunoglobulin G
|2 NLM
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| 650 |
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7 |
|a Oligodeoxyribonucleotides
|2 NLM
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| 650 |
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7 |
|a Spike Glycoprotein, Coronavirus
|2 NLM
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| 650 |
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7 |
|a spike protein, SARS-CoV-2
|2 NLM
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| 650 |
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7 |
|a DNA
|2 NLM
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| 650 |
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7 |
|a 9007-49-2
|2 NLM
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| 700 |
1 |
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|a Aljeraisi, Talal M
|e verfasserin
|4 aut
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| 773 |
0 |
8 |
|i Enthalten in
|t Clinical immunology (Orlando, Fla.)
|d 1999
|g 231(2021) vom: 01. Okt., Seite 108850
|w (DE-627)NLM098196855
|x 1521-7035
|7 nnns
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| 773 |
1 |
8 |
|g volume:231
|g year:2021
|g day:01
|g month:10
|g pages:108850
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| 856 |
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|u http://dx.doi.org/10.1016/j.clim.2021.108850
|3 Volltext
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|a GBV_USEFLAG_A
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|a SYSFLAG_A
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|a GBV_NLM
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|a GBV_ILN_11
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| 912 |
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|a GBV_ILN_24
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| 912 |
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|a GBV_ILN_350
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| 951 |
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|a AR
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| 952 |
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|d 231
|j 2021
|b 01
|c 10
|h 108850
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