Evidence of micro-evolution in Crocidura russula from two abandoned heavy metal mines : potential use of Cytb, CYP1A1, and p53 as gene biomarkers
© 2021. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.
| Veröffentlicht in: | Ecotoxicology (London, England). - 1992. - 30(2021), 10 vom: 10. Dez., Seite 1969-1982 |
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| Format: | Online-Aufsatz |
| Sprache: | English |
| Veröffentlicht: |
2021
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| Zugriff auf das übergeordnete Werk: | Ecotoxicology (London, England) |
| Schlagworte: | Journal Article Genetic diversity Heavy metal pollution Population effects Sentinel species Small mammals Biomarkers Metals, Heavy Tumor Suppressor Protein p53 Cytochromes b mehr... |
| Zusammenfassung: | © 2021. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature. Heavy metals accumulated in the environment due to the mining industry may impact on the health of exposed wild animals with consequences at the population level via survival and selection of the most resistant individuals. The detection and quantification of shifts in gene frequencies or in the genetic structure in populations inhabiting polluted sites may be used as early indicators of environmental stress and reveal potential 'candidate gene biomarkers' for environmental health assessment. We had previously observed that specimens of the Greater white-toothed shrew (Crocidura russula) from two heavy metal mines in Southern Portugal (the Aljustrel and the Preguiça mines) carried physiological alterations compared to shrews from an unpolluted site. Here, we further investigated whether these populations showed genetic differences in genes relevant for physiological homeostasis and/or that are associated with pathways altered in animals living under chronic exposure to pollution, and which could be used as biomarkers. We analysed the mitochondrial cytochrome b (Cytb) gene and intronic and/or exonic regions of four nuclear genes: CYP1A1, LCAT, PRPF31, and p53. We observed (1) population differences in allele frequencies, types of variation, and diversity parameters in the Cytb, CYP1A1, and p53 genes; (2) purifying selection of Cytb in the mine populations; (3) genetic differentiation of the two mine populations from the reference by the p53 gene. Adding to our previous observations with Mus spretus, we provide unequivocal evidence of a population effect exerted by the contaminated environment of the mines on the local species of small mammals |
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| Beschreibung: | Date Completed 23.11.2021 Date Revised 31.05.2022 published: Print-Electronic Citation Status MEDLINE |
| ISSN: | 1573-3017 |
| DOI: | 10.1007/s10646-021-02472-9 |