|
|
|
|
| LEADER |
01000naa a22002652 4500 |
| 001 |
NLM330371827 |
| 003 |
DE-627 |
| 005 |
20231225211138.0 |
| 007 |
cr uuu---uuuuu |
| 008 |
231225s2022 xx |||||o 00| ||eng c |
| 024 |
7 |
|
|a 10.1094/PDIS-07-21-1501-RE
|2 doi
|
| 028 |
5 |
2 |
|a pubmed24n1101.xml
|
| 035 |
|
|
|a (DE-627)NLM330371827
|
| 035 |
|
|
|a (NLM)34494869
|
| 040 |
|
|
|a DE-627
|b ger
|c DE-627
|e rakwb
|
| 041 |
|
|
|a eng
|
| 100 |
1 |
|
|a Li, Fang Jing
|e verfasserin
|4 aut
|
| 245 |
1 |
0 |
|a Molecular Diagnosis of Thiophanate-Methyl-Resistant Strains of Fusarium fujikuroi in Japan
|
| 264 |
|
1 |
|c 2022
|
| 336 |
|
|
|a Text
|b txt
|2 rdacontent
|
| 337 |
|
|
|a ƒaComputermedien
|b c
|2 rdamedia
|
| 338 |
|
|
|a ƒa Online-Ressource
|b cr
|2 rdacarrier
|
| 500 |
|
|
|a Date Completed 02.03.2022
|
| 500 |
|
|
|a Date Revised 02.03.2022
|
| 500 |
|
|
|a published: Print-Electronic
|
| 500 |
|
|
|a Citation Status MEDLINE
|
| 520 |
|
|
|a Fusarium fujikuroi is the pathogen of rice bakanae disease and is subclassified into gibberellin and fumonisin groups (G and F groups). Thiophanate-methyl (TM), a benzimidazole fungicide, has been used extensively to control F. fujikuroi. Previous investigation showed that F-group strains are TM sensitive (TMS), whereas most G-group strains are TM resistant (TMR) in Japan. The minimum inhibitory concentration in TMS strains was 1 to 10 μg ml-1, whereas that in TMR strains was >100 μg ml-1. E198K and F200Y mutations in β2-tubulin were detected in TMR strains. A loop-mediated isothermal amplification-fluorescent loop primer method was developed for diagnosis of these mutations and applied to 37 TMR strains and 56 TMS strains. The results indicated that 100% of TMR strains were identified as having either the E198K mutation (41%) or the F200Y mutation (59%), whereas none of the TMS strains tested showed either mutation. We found one remarkable TMR strain in the F group that had an F200Y mutation. These results suggest that E198K and F200Y mutations in β2-tubulin contribute to TM resistance in F. fujikuroi
|
| 650 |
|
4 |
|a Journal Article
|
| 650 |
|
4 |
|a Fusarium fujikuroi
|
| 650 |
|
4 |
|a LAMP-FLP
|
| 650 |
|
4 |
|a fungicide resistance mechanism
|
| 650 |
|
4 |
|a thiophanate-methyl
|
| 650 |
|
7 |
|a Fumonisins
|2 NLM
|
| 650 |
|
7 |
|a Thiophanate
|2 NLM
|
| 650 |
|
7 |
|a 5Q0Y96D5I8
|2 NLM
|
| 700 |
1 |
|
|a Komura, Ryoji
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Nakashima, Chiharu
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Shimizu, Masafumi
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Kageyama, Koji
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Suga, Haruhisa
|e verfasserin
|4 aut
|
| 773 |
0 |
8 |
|i Enthalten in
|t Plant disease
|d 1997
|g 106(2022), 2 vom: 31. Feb., Seite 634-640
|w (DE-627)NLM098181742
|x 0191-2917
|7 nnns
|
| 773 |
1 |
8 |
|g volume:106
|g year:2022
|g number:2
|g day:31
|g month:02
|g pages:634-640
|
| 856 |
4 |
0 |
|u http://dx.doi.org/10.1094/PDIS-07-21-1501-RE
|3 Volltext
|
| 912 |
|
|
|a GBV_USEFLAG_A
|
| 912 |
|
|
|a SYSFLAG_A
|
| 912 |
|
|
|a GBV_NLM
|
| 912 |
|
|
|a GBV_ILN_350
|
| 951 |
|
|
|a AR
|
| 952 |
|
|
|d 106
|j 2022
|e 2
|b 31
|c 02
|h 634-640
|