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231225s2021 xx |||||o 00| ||eng c |
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|a 10.1094/PDIS-03-21-0480-RE
|2 doi
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|a pubmed24n1099.xml
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|a (NLM)34455802
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|a DE-627
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|c DE-627
|e rakwb
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|a eng
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| 100 |
1 |
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|a Lv, Mingcan
|e verfasserin
|4 aut
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| 245 |
1 |
3 |
|a An Improved Technique for Isolation and Characterization of Single-Spore Isolates of Plasmodiophora brassicae
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| 264 |
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|c 2021
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| 336 |
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|a Text
|b txt
|2 rdacontent
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| 337 |
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|a ƒaComputermedien
|b c
|2 rdamedia
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| 338 |
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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| 500 |
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|a Date Completed 25.01.2022
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|a Date Revised 25.01.2022
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a Clubroot, caused by Plasmodiophora brassicae, is a soilborne disease that occurs in cruciferous crops worldwide. P. brassicae usually exists as a mixture of several pathotypes, which has hampered the research on resistance mechanisms of cruciferous crops against P. brassicae. In this study, clubroot galls were collected from a field in Shenyang, China, as a pathogen source to develop an efficient protocol for a single-spore isolation system of P. brassicae by optimizing the seedling age for inoculation, host inoculation method, and plant culture method. The operational steps of the single-spore isolation method were optimized as follows: the use of 2-day-old seedlings for inoculation, substituting a cryobox (100 × 2.0-ml vials) for culture dishes, the addition of nutrient solution culture, and microscopic observations of single spores. The rate of infection success was substantially improved, and single-spore isolates of four pathotypes (4, 8, 9, and 11) were acquired in this system. Subsequently, the optimized system was used to isolate and characterize the pathotypes of single-spore isolates of P. brassicae collected from five fields in regions in China. Approximately four to nine pathotypes were isolated from each region. Among these, pathotype 4 was the most prevalent. This study provides a source of valuable information that can eventually be used for the genetic analysis of host-P. brassicae interaction
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4 |
|a Journal Article
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4 |
|a Cruciferae crops
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| 650 |
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4 |
|a Plasmodiophora brassicae
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| 650 |
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4 |
|a pathotypes
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| 650 |
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4 |
|a single-spore isolate characterization
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| 700 |
1 |
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|a Liu, Yifan
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Wu, Yue
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Zhang, Jing
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Liu, Xuyao
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Ji, Ruiqin
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Feng, Hui
|e verfasserin
|4 aut
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| 773 |
0 |
8 |
|i Enthalten in
|t Plant disease
|d 1997
|g 105(2021), 12 vom: 08. Dez., Seite 3932-3938
|w (DE-627)NLM098181742
|x 0191-2917
|7 nnns
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| 773 |
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|g volume:105
|g year:2021
|g number:12
|g day:08
|g month:12
|g pages:3932-3938
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|u http://dx.doi.org/10.1094/PDIS-03-21-0480-RE
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