Optimization of phosphate-limited autoinduction broth for two-stage heterologous protein expression in Escherichia coli
Autoinducible, two-stage protein expression leveraging phosphate-inducible promoters has been recently shown to enable not only high protein titers but also consistent performance across scales from screening systems (microtiter plates) to instrumented bioreactors. However, to date, small-scale prod...
| Veröffentlicht in: | BioTechniques. - 1993. - 71(2021), 5 vom: 07. Nov., Seite 566-572 |
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| Format: | Online-Aufsatz |
| Sprache: | English |
| Veröffentlicht: |
2021
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| Zugriff auf das übergeordnete Werk: | BioTechniques |
| Schlagworte: | Journal Article Research Support, Non-U.S. Gov't autoinduction media phosphate induction protein expression Culture Media Phosphates Recombinant Proteins |
| Online verfügbar |
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