Two evolutionarily duplicated domains individually and post-transcriptionally control SWEET expression for phloem transport

Two evolutionarily duplicated domains individually and post-transcriptionally control SWEET expression for phloem transport

© 2021 The Authors. New Phytologist © 2021 New Phytologist Foundation.

Bibliographische Detailangaben
Veröffentlicht in:The New phytologist. - 1979. - 232(2021), 4 vom: 25. Nov., Seite 1793-1807
1. Verfasser: Zhang, Chen (VerfasserIn)
Weitere Verfasser: Li, Ye, Wang, Jiang, Xue, Xueyi, Beuchat, Gabriel, Chen, Li-Qing
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2021
Zugriff auf das übergeordnete Werk:The New phytologist
Schlagworte:Journal Article Research Support, Non-U.S. Gov't SWEET transporter coevolution phloem parenchyma post-transcriptional regulation specific expression vascular plants Arabidopsis Proteins Membrane Transport Proteins mehr... Plant Proteins SWEET11 protein, Arabidopsis SWEET12 protein, Arabidopsis
Beschreibung
Zusammenfassung:© 2021 The Authors. New Phytologist © 2021 New Phytologist Foundation.
Cell type-specific gene expression is critical for the specialized functions within multicellular organisms. In Arabidopsis, SWEET11 and SWEET12 sugar transporters are specifically expressed in phloem parenchyma (PP) cells and are responsible for sucrose efflux from the PP, the first step of a two-step apoplasmic phloem-loading strategy that initiates the long-distance transport of sugar from leaves to nonphotosynthetic sink tissues. However, we know nothing about what determines the PP cell-specific expression of these SWEETs. Sequence deletions, histochemical β-glucuronidase (GUS) analysis, cross-sectioning, live-cell imaging, and evolutionary analysis were used to elucidate domains responsible for PP specificity, while a Förster resonance energy transfer (FRET) sensor-based transport assay was used to determine whether substrate specificity coevolved with PP specificity. We identified two domains in the Arabidopsis SWEET11 coding sequence that, along with its promoter (including 5' UTR), regulate PP-specific expression at the post-transcriptional level, probably involving RNA-binding proteins. This mechanism is conserved among vascular plants but independent of transport substrate specificity. We conclude that two evolutionarily duplicated coding sequence domains are essential and individually sufficient for PP-specific expression of SWEET11. We also provide a crucial experimental tool to study PP physiology and development
Beschreibung:Date Completed 27.10.2021
Date Revised 31.05.2022
published: Print-Electronic
Citation Status MEDLINE
ISSN:1469-8137
DOI:10.1111/nph.17688