Deciphering ploidal levels of Lippia alba by using proteomics

Copyright © 2021 Elsevier Masson SAS. All rights reserved.

Bibliographische Detailangaben
Veröffentlicht in:Plant physiology and biochemistry : PPB. - 1991. - 167(2021) vom: 30. Okt., Seite 385-389
1. Verfasser: de Souza, Camila Maurmann (VerfasserIn)
Weitere Verfasser: Zorzatto, Cristiane, Quinhones, Carla G S, Lopes, Juliana Mainenti Leal, de Carvalho, Humberto Henrique, Araújo, Wagner L, Viccini, Lyderson Facio
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2021
Zugriff auf das übergeordnete Werk:Plant physiology and biochemistry : PPB
Schlagworte:Journal Article Essential oil Medicinal plants Phytotherapy Polyploidy Proteome Tropics Whole-genome duplication Proteins
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520 |a Lippia alba (Mill.) N.E. Brown (Verbenaceae), popularly known as "lemon balm" or "bushy matgrass", is widely used in folk medicine due to its anti-inflammatory, antispasmodic, analgesic, and digestive properties. It was described as an autopolyploid complex with five cytotypes (2n = 30, 38, 45, 60 and 90). To enhance our understanding of the biological variation of the species, we investigated, comparatively, the proteomic profile of all ploidal levels (diploid, aneuploid, triploid, tetraploid, and hexaploid). Leaf proteins were extracted with subsequent separation by two-dimensional electrophoresis, spot analysis, and protein identification by mass spectrometry. By comparing the proteomic profile of diploid accession to the profile of the other ploidal levels we identified differential expression between the analysed spots. We identified 34 proteins with differential expression between the ploidal levels in comparison with the diploid. The identified proteins seem to play relevant roles in the primary metabolism of L. alba suggesting that a specific set of proteins was selected during the polyploidization process, being the triploid the most different one. Given that protein composition can substantially affect the desired therapeutic effect, we posit that further combination of proteomic and metabolomic studies may help to unravel genetic variations and phenotypic profiles in L. alba 
650 4 |a Journal Article 
650 4 |a Essential oil 
650 4 |a Medicinal plants 
650 4 |a Phytotherapy 
650 4 |a Polyploidy 
650 4 |a Proteome 
650 4 |a Tropics 
650 4 |a Whole-genome duplication 
650 7 |a Proteins  |2 NLM 
700 1 |a Zorzatto, Cristiane  |e verfasserin  |4 aut 
700 1 |a Quinhones, Carla G S  |e verfasserin  |4 aut 
700 1 |a Lopes, Juliana Mainenti Leal  |e verfasserin  |4 aut 
700 1 |a de Carvalho, Humberto Henrique  |e verfasserin  |4 aut 
700 1 |a Araújo, Wagner L  |e verfasserin  |4 aut 
700 1 |a Viccini, Lyderson Facio  |e verfasserin  |4 aut 
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773 1 8 |g volume:167  |g year:2021  |g day:30  |g month:10  |g pages:385-389 
856 4 0 |u http://dx.doi.org/10.1016/j.plaphy.2021.08.018  |3 Volltext 
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