Identification of cis conformation natural rubber and proteins in Ficus altissima Blume latex
Copyright © 2021 Elsevier Masson SAS. All rights reserved.
| Veröffentlicht in: | Plant physiology and biochemistry : PPB. - 1991. - 167(2021) vom: 30. Okt., Seite 376-384 |
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| Weitere Verfasser: | , , |
| Format: | Online-Aufsatz |
| Sprache: | English |
| Veröffentlicht: |
2021
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| Zugriff auf das übergeordnete Werk: | Plant physiology and biochemistry : PPB |
| Schlagworte: | Journal Article Ficus altissima Latex Natural rubber Protein Rubber particle Plant Proteins Rubber 9006-04-6 |
| Zusammenfassung: | Copyright © 2021 Elsevier Masson SAS. All rights reserved. Ficus altissima Blume, a horticultural plant in tropical and warm subtropical regions, can produce natural rubber with comparable molecular weight to the Hevea brasiliensis rubber. The F. altissima latex has an acidic pH (about 4.89). The rubber particle size distribution is a unimodal profile, and the peak frequency is at a size of 4.5 μm. The natural rubber of F. altissima was determined to be a cis conformation via 13C NMR. The Mp (molecular weight of the peak maxima) of the deproteinized F. altissima rubber was 9.34 × 105 Da. LC-MS was used to identify the proteins of rubber particles and serum. The most abundant protein of the creamy rubber particle layer is an acid phosphatase, while the most abundant proteins of serum were an (R)-mandelonitrilelyase and a polygalacturonase inhibitor. Pharmaceutical proteins (ficins) or enzymes related to the biosynthesis of natural medicines (a cannabidiolic acid synthase and two lupeol synthase) were identified in F. altissima latex. The data of this study may be helpful for research on the functions of latex in latex-borne plants and the biosynthesis mechanism of natural rubber |
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| Beschreibung: | Date Completed 13.10.2021 Date Revised 13.10.2021 published: Print-Electronic Citation Status MEDLINE |
| ISSN: | 1873-2690 |
| DOI: | 10.1016/j.plaphy.2021.08.015 |