Simple, inexpensive and RNase-free purification of plasmid DNA by fractional precipitation with isopropanol
We present a modified alkaline lysis method for purification of plasmid DNA (pDNA) from bacterial extract using fractional precipitation with isopropanol (FPI). This method includes two successive precipitations with 0.33 and 0.36 volumes of isopropanol and separates pDNA from total RNA and most of...
| Veröffentlicht in: | BioTechniques. - 1988. - 71(2021), 3 vom: 16. Sept., Seite 490-494 |
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| Format: | Online-Aufsatz |
| Sprache: | English |
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2021
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| Zugriff auf das übergeordnete Werk: | BioTechniques |
| Schlagworte: | Journal Article Research Support, Non-U.S. Gov't RNase alkaline lysis fractional precipitation isopropanol lipopolysaccharides plasmid DNA DNA, Bacterial Ribonucleases mehr... |
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