Treatment of acid rock drainage using a sulphate-reducing bioreactor with a limestone precolumn

Sulphate reducing bacteria (SRB) offer promise for the treatment of mine waste due to their effectiveness removing toxic heavy metals as highly insoluble metal sulphides and their ability to generate alkalinity. The main objective of this study was to develop a treatment composed of a sulphate-reduc...

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Veröffentlicht in:Environmental technology. - 1993. - 44(2023), 2 vom: 09. Jan., Seite 185-196
1. Verfasser: Méndez, Gabriela (VerfasserIn)
Weitere Verfasser: Trueba, Gabriel, Sierra-Alvarez, Reyes, Ochoa-Herrera, Valeria
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2023
Zugriff auf das übergeordnete Werk:Environmental technology
Schlagworte:Journal Article Sulphate-reducing bacteria acetate acid rock drainage copper methanogens Metals, Heavy Sulfates Calcium Carbonate H0G9379FGK Acetates
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520 |a Sulphate reducing bacteria (SRB) offer promise for the treatment of mine waste due to their effectiveness removing toxic heavy metals as highly insoluble metal sulphides and their ability to generate alkalinity. The main objective of this study was to develop a treatment composed of a sulphate-reducing bioreactor with a limestone precolumn for the removal of Cu(II) from a synthetic ARD. The purpose of the limestone column was to increase the pH values and decrease the level of Cu in the effluent to prevent SRB inhibition. The system was fed with a pH-2.7 synthetic ARD containing Cu(II) (10-40 mg/L), sulphate (2000 mg/L) and acetate (2.5 g COD/L) for 150 days. Copper removal efficiencies in the two-stage system were very high (95-99%), with a final concentration of 0.53 mg/L Cu, and almost complete removal occurred in the limestone precolumn. In the same manner, the acidity of the synthetic ARD was effectively reduced in the limestone precolumn to 7.3 and the pH was raised in the bioreactor (7.3-8.0). COD consumption by methanogens was predominant from day 0-118, but SRB dominated at the end of the experiment (day 150) when the average COD removal and sulphide production were 74.8% and 61.7%, respectively. Study of the microbial taxonomic composition in the bioreactor revealed that Methanosarcina and Methanosaeta were the most prevalent methanogens while the genera Desulfotomaculum and Syntrophobacter were the dominant SRB. Among the SRB identified Desulfotomaculum intricatum (99% identity) and Desulfotomaculum acetoxidans (96%) were the most abundant sequences of bacteria capable of using acetate 
650 4 |a Journal Article 
650 4 |a Sulphate-reducing bacteria 
650 4 |a acetate 
650 4 |a acid rock drainage 
650 4 |a copper 
650 4 |a methanogens 
650 7 |a Metals, Heavy  |2 NLM 
650 7 |a Sulfates  |2 NLM 
650 7 |a Calcium Carbonate  |2 NLM 
650 7 |a H0G9379FGK  |2 NLM 
650 7 |a Acetates  |2 NLM 
700 1 |a Trueba, Gabriel  |e verfasserin  |4 aut 
700 1 |a Sierra-Alvarez, Reyes  |e verfasserin  |4 aut 
700 1 |a Ochoa-Herrera, Valeria  |e verfasserin  |4 aut 
773 0 8 |i Enthalten in  |t Environmental technology  |d 1993  |g 44(2023), 2 vom: 09. Jan., Seite 185-196  |w (DE-627)NLM098202545  |x 1479-487X  |7 nnns 
773 1 8 |g volume:44  |g year:2023  |g number:2  |g day:09  |g month:01  |g pages:185-196 
856 4 0 |u http://dx.doi.org/10.1080/09593330.2021.1968039  |3 Volltext 
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