DNA marker for resistance to Puccinia horiana in chrysanthemum (Chrysanthemum morifolium Ramat.) "Southern Pegasus"
Copyright © 2021 by JAPANESE SOCIETY OF BREEDING.
| Veröffentlicht in: | Breeding science. - 1998. - 71(2021), 2 vom: 15. Apr., Seite 261-267 |
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| 1. Verfasser: | |
| Weitere Verfasser: | , , , , , , , , |
| Format: | Online-Aufsatz |
| Sprache: | English |
| Veröffentlicht: |
2021
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| Zugriff auf das übergeordnete Werk: | Breeding science |
| Schlagworte: | Journal Article chrysanthemum white rust genome-wide association study simplex single nucleotide polymorphism marker |
| Zusammenfassung: | Copyright © 2021 by JAPANESE SOCIETY OF BREEDING. White rust caused by Puccinia horiana Henn. adversely affects chrysanthemum (Chrysanthemum morifolium Ramat.) production. The breeding of resistant varieties is effective in controlling the disease. Here we aimed to develop DNA markers for the strong resistance to P. horiana. We conducted a linkage analysis based on the genome-wide association study (GWAS) method. We employed a biparental population for the GWAS, wherein the single nucleotide polymorphism (SNP) allele frequency could be predicted. The population was derived from crosses between a strong resistant "Southern Pegasus" and a susceptible line. The GWAS used simplex and double-simplex SNP markers selected out of SNP candidates mined from ddRAD-Seq data of an F1 biparental population. These F1 individuals segregated in a 1:1 ratio of resistant to susceptible. Twenty-one simplex SNPs were significantly associated with P. horiana resistance in "Southern Pegasus" and generated one linkage group. These results show the presence of a single resistance gene in "Southern Pegasus". We identified the nearest SNP marker located 2.2 cM from P. horiana resistance locus and demonstrated this SNP marker-resistance link using an independent population. This is the first report of an effective DNA marker linked to a gene for P. horiana resistance in chrysanthemum |
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| Beschreibung: | Date Revised 12.08.2021 published: Print-Electronic Citation Status PubMed-not-MEDLINE |
| ISSN: | 1344-7610 |
| DOI: | 10.1270/jsbbs.20063 |