Development of an SSR marker set for efficient selection for resistance to black spot disease in pear breeding

Copyright © 2021 by JAPANESE SOCIETY OF BREEDING.

Bibliographische Detailangaben
Veröffentlicht in:Breeding science. - 1998. - 71(2021), 2 vom: 15. Apr., Seite 240-252
1. Verfasser: Terakami, Shingo (VerfasserIn)
Weitere Verfasser: Adachi, Yoshihiko, Takeuchi, Yukie, Takada, Norio, Nishio, Sogo, Saito, Toshihiro, Yamamoto, Toshiya
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2021
Zugriff auf das übergeordnete Werk:Breeding science
Schlagworte:Journal Article Alternaria alternata Pyruspyrifolia Pyrusussuriensis black spot disease marker-assisted selection
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520 |a Black spot disease, which is caused by Alternaria alternata (Fries) Keissler Japanese pear pathotype, is one of the most harmful diseases in Japanese pear cultivation. Because of the potential harm of fungicides to consumers and the environment, resistant cultivars are desired. In this study, to enable efficient marker-assisted selection in pear breeding, we conducted comprehensive inoculation tests and genotyping with 207 pear cultivars. We identified a marker set (Mdo.chr11.27 and Mdo.chr11.34) suitable for selection for black spot resistance. In most susceptible cultivars, Mdo.chr11.27 amplified a 220-bp band and Mdo.chr11.34 amplified a 259-bp band. The genotype of Mdo.chr11.34 corresponds perfectly to the estimated genotype of Japanese pears susceptible to black spot disease. Using linkage analysis, we identified the positions of the gene for susceptibility to black spot disease in Chinese pear. Mdo.chr11.27 and Mdo.chr11.34 were tightly linked to susceptibility in Chinese pear, and the susceptibility gene was mapped at the top of linkage group 11, similar to that in Japanese pear. This marker set and the accumulation of phenotypic data will enable efficient marker-assisted breeding for black spot resistance in pear breeding 
650 4 |a Journal Article 
650 4 |a Alternaria alternata 
650 4 |a Pyruspyrifolia 
650 4 |a Pyrusussuriensis 
650 4 |a black spot disease 
650 4 |a marker-assisted selection 
700 1 |a Adachi, Yoshihiko  |e verfasserin  |4 aut 
700 1 |a Takeuchi, Yukie  |e verfasserin  |4 aut 
700 1 |a Takada, Norio  |e verfasserin  |4 aut 
700 1 |a Nishio, Sogo  |e verfasserin  |4 aut 
700 1 |a Saito, Toshihiro  |e verfasserin  |4 aut 
700 1 |a Yamamoto, Toshiya  |e verfasserin  |4 aut 
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773 1 8 |g volume:71  |g year:2021  |g number:2  |g day:15  |g month:04  |g pages:240-252 
856 4 0 |u http://dx.doi.org/10.1270/jsbbs.20136  |3 Volltext 
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