Single-cell RNA sequencing of intramedullary canal tissue to improve methods for studying fracture repair biology

The ability to study the bone microenvironment of failed fracture healing may lead to biomarkers for fracture nonunion. Herein the authors describe a technique for isolating individual cells suitable for single-cell RNA sequencing analyses from intramedullary canal tissue collected by reaming during...

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Veröffentlicht in:	BioTechniques. - 1988. - 71(2021), 2 vom: 01. Aug., Seite 431-438
1. Verfasser:	Dominguez, James M 2nd (VerfasserIn)
Weitere Verfasser:	Moe, Sharon M, Chen, Neal X, McKinley, Todd O, Brown, Krista M, Liu, Yunlong, Gao, Hongyu, Natoli, Roman M
Format:	Online-Aufsatz
Sprache:	English
Veröffentlicht:	2021
Zugriff auf das übergeordnete Werk:	BioTechniques
Schlagworte:	Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't cell isolation cryopreservation fracture nonunion single-cell RNA sequencing