Single-cell RNA sequencing of intramedullary canal tissue to improve methods for studying fracture repair biology

The ability to study the bone microenvironment of failed fracture healing may lead to biomarkers for fracture nonunion. Herein the authors describe a technique for isolating individual cells suitable for single-cell RNA sequencing analyses from intramedullary canal tissue collected by reaming during...

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Détails bibliographiques
Publié dans:BioTechniques. - 1991. - 71(2021), 2 vom: 01. Aug., Seite 431-438
Auteur principal: Dominguez, James M 2nd (Auteur)
Autres auteurs: Moe, Sharon M, Chen, Neal X, McKinley, Todd O, Brown, Krista M, Liu, Yunlong, Gao, Hongyu, Natoli, Roman M
Format: Article en ligne
Langue:English
Publié: 2021
Accès à la collection:BioTechniques
Sujets:Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't cell isolation cryopreservation fracture nonunion single-cell RNA sequencing