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231225s2021 xx |||||o 00| ||eng c |
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|a 10.1021/acs.langmuir.1c01587
|2 doi
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|a pubmed24n1097.xml
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|a (NLM)34369787
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|a DE-627
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|a eng
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| 100 |
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|a Astolfi, Paola
|e verfasserin
|4 aut
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|a Cubic and Hexagonal Mesophases for Protein Encapsulation
|b Structural Effects of Insulin Confinement
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|c 2021
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
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|a Date Completed 26.08.2021
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|a Date Revised 01.09.2021
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a Monoolein-based cubic and hexagonal mesophases were investigated as matrices for insulin loading, at low pH, as a function of temperature and in the presence of increasing amounts of oleic acid, as a structural stabilizer for the hexagonal phase. Synchrotron small angle X-ray diffraction, rheological measurements, and attenuated total reflection-Fourier transform infrared spectroscopy were used to study the effects of insulin loading on the lipid mesophases and of the effect of protein confinement in the 2D- and 3D-lipid matrix water channels on its stability and unfolding behavior. We found that insulin encapsulation has only little effects both on the mesophase structures and on the viscoelastic properties of lipid systems, whereas protein confinement affects the response of the secondary structure of insulin to thermal changes in a different manner according to the specific mesophase: in the cubic structure, the unfolding toward an unordered structure is favored, while the prevalence of parallel β-sheets, and nuclei for fibril formation, is observed in hexagonal structures
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a Insulin
|2 NLM
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|a Giorgini, Elisabetta
|e verfasserin
|4 aut
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|a Perinelli, Diego Romano
|e verfasserin
|4 aut
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| 700 |
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|a Vita, Francesco
|e verfasserin
|4 aut
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|a Adamo, Fabrizio Corrado
|e verfasserin
|4 aut
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| 700 |
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|a Logrippo, Serena
|e verfasserin
|4 aut
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| 700 |
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|a Parlapiano, Marco
|e verfasserin
|4 aut
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|a Bonacucina, Giulia
|e verfasserin
|4 aut
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| 700 |
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|a Pucciarelli, Stefania
|e verfasserin
|4 aut
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| 700 |
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|a Francescangeli, Oriano
|e verfasserin
|4 aut
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| 700 |
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|a Vaccari, Lisa
|e verfasserin
|4 aut
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| 700 |
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|a Pisani, Michela
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Langmuir : the ACS journal of surfaces and colloids
|d 1992
|g 37(2021), 33 vom: 24. Aug., Seite 10166-10176
|w (DE-627)NLM098181009
|x 1520-5827
|7 nnns
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|g volume:37
|g year:2021
|g number:33
|g day:24
|g month:08
|g pages:10166-10176
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|u http://dx.doi.org/10.1021/acs.langmuir.1c01587
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