|
|
|
|
| LEADER |
01000caa a22002652 4500 |
| 001 |
NLM328918873 |
| 003 |
DE-627 |
| 005 |
20240403232623.0 |
| 007 |
cr uuu---uuuuu |
| 008 |
231225s2021 xx |||||o 00| ||eng c |
| 024 |
7 |
|
|a 10.1021/acs.langmuir.1c01410
|2 doi
|
| 028 |
5 |
2 |
|a pubmed24n1362.xml
|
| 035 |
|
|
|a (DE-627)NLM328918873
|
| 035 |
|
|
|a (NLM)34347486
|
| 040 |
|
|
|a DE-627
|b ger
|c DE-627
|e rakwb
|
| 041 |
|
|
|a eng
|
| 100 |
1 |
|
|a Morales-García, Ana L
|e verfasserin
|4 aut
|
| 245 |
1 |
4 |
|a The Role of Extracellular DNA in Microbial Attachment to Oxidized Silicon Surfaces in the Presence of Ca2+ and Na
|
| 264 |
|
1 |
|c 2021
|
| 336 |
|
|
|a Text
|b txt
|2 rdacontent
|
| 337 |
|
|
|a ƒaComputermedien
|b c
|2 rdamedia
|
| 338 |
|
|
|a ƒa Online-Ressource
|b cr
|2 rdacarrier
|
| 500 |
|
|
|a Date Revised 03.04.2024
|
| 500 |
|
|
|a published: Print-Electronic
|
| 500 |
|
|
|a Citation Status Publisher
|
| 520 |
|
|
|a Attachment assays of a Pseudomonas isolate to fused silica slides showed that treatment with DNaseI significantly inhibited cellular adsorption, which was restored upon DNA treatment. These assays confirmed the important role of extracellular DNA (eDNA) adsorption to a surface. To investigate the eDNA adsorption mechanism, single-molecule force spectroscopy (SMFS) was used to measure the adsorption of eDNA to silicon surfaces in the presence of different concentrations of sodium and calcium ions. SMFS reveals that the work of adhesion required to remove calcium-bound eDNA from the silicon oxide surface is substantially greater than that for sodium. Molecular dynamics simulations were also performed, and here, it was shown that the energy gain in eDNA adsorption to a silicon oxide surface in the presence of calcium ions is small and much less than that in the presence of sodium. The simulations show that the length scales involved in eDNA adsorption are less in the presence of sodium ions than those in the presence of calcium. In the presence of calcium, eDNA is pushed above the surface cations, whereas in the presence of sodium ions, short-range interactions with the surface dominate. Moreover, SMFS data show that increasing [Ca2+] from 1 to 10 mM increases the adsorption of the cations to the silicon oxide surface and consequently enhances the Stern layer, which in turn increases the length scale associated with eDNA adsorption
|
| 650 |
|
4 |
|a Journal Article
|
| 700 |
1 |
|
|a Walton, Rachel
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Blakeman, Jamie T
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Banwart, Steven A
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Harding, John H
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Geoghegan, Mark
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Freeman, Colin L
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Rolfe, Stephen A
|e verfasserin
|4 aut
|
| 773 |
0 |
8 |
|i Enthalten in
|t Langmuir : the ACS journal of surfaces and colloids
|d 1999
|g (2021) vom: 04. Aug.
|w (DE-627)NLM098181009
|x 1520-5827
|7 nnns
|
| 773 |
1 |
8 |
|g year:2021
|g day:04
|g month:08
|
| 856 |
4 |
0 |
|u http://dx.doi.org/10.1021/acs.langmuir.1c01410
|3 Volltext
|
| 912 |
|
|
|a GBV_USEFLAG_A
|
| 912 |
|
|
|a SYSFLAG_A
|
| 912 |
|
|
|a GBV_NLM
|
| 912 |
|
|
|a GBV_ILN_22
|
| 912 |
|
|
|a GBV_ILN_350
|
| 912 |
|
|
|a GBV_ILN_721
|
| 951 |
|
|
|a AR
|
| 952 |
|
|
|j 2021
|b 04
|c 08
|