Evolutionarily distinct resistance proteins detect a pathogen effector through its association with different host targets

Evolutionarily distinct resistance proteins detect a pathogen effector through its association with different host targets

© 2021 The Authors. New Phytologist © 2021 New Phytologist Foundation.

Bibliographische Detailangaben
Veröffentlicht in:The New phytologist. - 1979. - 232(2021), 3 vom: 01. Nov., Seite 1368-1381
1. Verfasser: Wang, Haixia (VerfasserIn)
Weitere Verfasser: Trusch, Franziska, Turnbull, Dionne, Aguilera-Galvez, Carolina, Breen, Susan, Naqvi, Shaista, Jones, Jonathan D G, Hein, Ingo, Tian, Zhendong, Vleeshouwers, Vivianne, Gilroy, Eleanor, Birch, Paul R J
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2021
Zugriff auf das übergeordnete Werk:The New phytologist
Schlagworte:Journal Article Research Support, Non-U.S. Gov't NLR avirulence cell death effector-triggered immunity plant immunity plant pathogen co-evolution potato late blight resistance protein Plant Proteins
LEADER 01000naa a22002652 4500
001 NLM328840319
003 DE-627
005 20231225203817.0
007 cr uuu---uuuuu
008 231225s2021 xx |||||o 00| ||eng c
024 7 |a 10.1111/nph.17660  |2 doi 
028 5 2 |a pubmed24n1096.xml 
035 |a (DE-627)NLM328840319 
035 |a (NLM)34339518 
040 |a DE-627  |b ger  |c DE-627  |e rakwb 
041 |a eng 
100 1 |a Wang, Haixia  |e verfasserin  |4 aut 
245 1 0 |a Evolutionarily distinct resistance proteins detect a pathogen effector through its association with different host targets 
264 1 |c 2021 
336 |a Text  |b txt  |2 rdacontent 
337 |a ƒaComputermedien  |b c  |2 rdamedia 
338 |a ƒa Online-Ressource  |b cr  |2 rdacarrier 
500 |a Date Completed 18.10.2021 
500 |a Date Revised 10.03.2022 
500 |a published: Print-Electronic 
500 |a Citation Status MEDLINE 
520 |a © 2021 The Authors. New Phytologist © 2021 New Phytologist Foundation. 
520 |a Knowledge of the evolutionary processes which govern pathogen recognition is critical to understanding durable disease resistance. We determined how Phytophthora infestans effector PiAVR2 is recognised by evolutionarily distinct resistance proteins R2 and Rpi-mcq1. We employed yeast two-hybrid, co-immunoprecipitation, virus-induced gene silencing, transient overexpression, and phosphatase activity assays to investigate the contributions of BSL phosphatases to R2- and Rpi-mcq1-mediated hypersensitive response (R2 HR and Rpi-mcq1 HR, respectively). Silencing PiAVR2 target BSL1 compromises R2 HR. Rpi-mcq1 HR is compromised only when BSL2 and BSL3 are silenced. BSL1 overexpression increases R2 HR and compromises Rpi-mcq1. However, overexpression of BSL2 or BSL3 enhances Rpi-mcq1 and compromises R2 HR. Okadaic acid, which inhibits BSL phosphatase activity, suppresses both recognition events. Moreover, expression of a BSL1 phosphatase-dead (PD) mutant suppresses R2 HR, whereas BSL2-PD and BSL3-PD mutants suppress Rpi-mcq1 HR. R2 interacts with BSL1 in the presence of PiAVR2, but not with BSL2 and BSL3, whereas no interactions were detected between Rpi-mcq1 and BSLs. Thus, BSL1 activity and association with R2 determine recognition of PiAVR2 by R2, whereas BSL2 and BSL3 mediate Rpi-mcq1 perception of PiAVR2. R2 and Rpi-mcq1 utilise distinct mechanisms to detect PiAVR2 based on association with different BSLs, highlighting central roles of these effector targets for both disease and disease resistance 
650 4 |a Journal Article 
650 4 |a Research Support, Non-U.S. Gov't 
650 4 |a NLR 
650 4 |a avirulence 
650 4 |a cell death 
650 4 |a effector-triggered immunity 
650 4 |a plant immunity 
650 4 |a plant pathogen co-evolution 
650 4 |a potato late blight 
650 4 |a resistance protein 
650 7 |a Plant Proteins  |2 NLM 
700 1 |a Trusch, Franziska  |e verfasserin  |4 aut 
700 1 |a Turnbull, Dionne  |e verfasserin  |4 aut 
700 1 |a Aguilera-Galvez, Carolina  |e verfasserin  |4 aut 
700 1 |a Breen, Susan  |e verfasserin  |4 aut 
700 1 |a Naqvi, Shaista  |e verfasserin  |4 aut 
700 1 |a Jones, Jonathan D G  |e verfasserin  |4 aut 
700 1 |a Hein, Ingo  |e verfasserin  |4 aut 
700 1 |a Tian, Zhendong  |e verfasserin  |4 aut 
700 1 |a Vleeshouwers, Vivianne  |e verfasserin  |4 aut 
700 1 |a Gilroy, Eleanor  |e verfasserin  |4 aut 
700 1 |a Birch, Paul R J  |e verfasserin  |4 aut 
773 0 8 |i Enthalten in  |t The New phytologist  |d 1979  |g 232(2021), 3 vom: 01. Nov., Seite 1368-1381  |w (DE-627)NLM09818248X  |x 1469-8137  |7 nnns 
773 1 8 |g volume:232  |g year:2021  |g number:3  |g day:01  |g month:11  |g pages:1368-1381 
856 4 0 |u http://dx.doi.org/10.1111/nph.17660  |3 Volltext 
912 |a GBV_USEFLAG_A 
912 |a SYSFLAG_A 
912 |a GBV_NLM 
912 |a GBV_ILN_350 
951 |a AR 
952 |d 232  |j 2021  |e 3  |b 01  |c 11  |h 1368-1381