Integrative analysis of GWAS and transcriptome to reveal novel loci regulation flowering time in semi-winter rapeseed

Copyright © 2021 Elsevier B.V. All rights reserved.

Bibliographische Detailangaben
Veröffentlicht in:Plant science : an international journal of experimental plant biology. - 1985. - 310(2021) vom: 15. Sept., Seite 110980
1. Verfasser: Huang, Luyao (VerfasserIn)
Weitere Verfasser: Min, Yao, Schiessl, Sarah, Xiong, Xinghua, Jan, Habib U, He, Xin, Qian, Wei, Guan, Chunyun, Snowdon, Rod J, Hua, Wei, Guan, Mei, Qian, Lunwen
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2021
Zugriff auf das übergeordnete Werk:Plant science : an international journal of experimental plant biology
Schlagworte:Journal Article Brassica napus Coexpression network Flowering time Genome-wide association analysis Haplotype Plant Proteins
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520 |a Flowering is an important turning point from vegetative growth to reproductive growth, and vernalization is an essential condition for the flowering of annual winter plants. To investigate the genetic architecture of flowering time in rapeseed, we used the 60 K Brassica Infinium SNP array to perform a genome-wide analysis of haplotype blocks associated with flowering time in 203 Chinese semi-winter rapeseed inbred lines. Twenty-one haplotype regions carrying one or more candidate genes showed a significant association with flowering time. Interestingly, we detected a SNP (Bn-scaff_22728_1-p285715) located in exon 3 of the BnVIN3-C03 gene that showed a significant association with flowering time on chromosome C03. Based on the SNP alleles A and G, two groups of accessions with early and late flowering time phenotypes were selected, respectively, and PCR amplification and gene expression analysis were combined to reveal the structural variation of the BnVIN3-C03 gene that affected flowering time. Moreover, we found that BnVIN3-C03 inhibited the expression of BnFLC-A02, BnFLC-A03.1, BnFLC-A10 and BnFLC-C03.1, thus modulating the flowering time of Brassica napus. This result provides insight into the genetic improvement of flowering time in B. napus 
650 4 |a Journal Article 
650 4 |a Brassica napus 
650 4 |a Coexpression network 
650 4 |a Flowering time 
650 4 |a Genome-wide association analysis 
650 4 |a Haplotype 
650 7 |a Plant Proteins  |2 NLM 
700 1 |a Min, Yao  |e verfasserin  |4 aut 
700 1 |a Schiessl, Sarah  |e verfasserin  |4 aut 
700 1 |a Xiong, Xinghua  |e verfasserin  |4 aut 
700 1 |a Jan, Habib U  |e verfasserin  |4 aut 
700 1 |a He, Xin  |e verfasserin  |4 aut 
700 1 |a Qian, Wei  |e verfasserin  |4 aut 
700 1 |a Guan, Chunyun  |e verfasserin  |4 aut 
700 1 |a Snowdon, Rod J  |e verfasserin  |4 aut 
700 1 |a Hua, Wei  |e verfasserin  |4 aut 
700 1 |a Guan, Mei  |e verfasserin  |4 aut 
700 1 |a Qian, Lunwen  |e verfasserin  |4 aut 
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