Ultrasensitive Immunosensor Based on Langmuir-Blodgett Deposited Ordered Graphene Assemblies for Dengue Detection

In this manuscript partially reduced graphene oxide (RGO) nanosheet-based electrodes have been utilized for quantification of the NS1 protein and subsequently for dengue detection. NS1 is the biomarker found circulating in the body of dengue-infected persons on or after first day of the appearance o...

Ausführliche Beschreibung

Bibliographische Detailangaben
Veröffentlicht in:Langmuir : the ACS journal of surfaces and colloids. - 1992. - 37(2021), 29 vom: 27. Juli, Seite 8705-8713
1. Verfasser: Solanki, Shipra (VerfasserIn)
Weitere Verfasser: Soni, Amrita, Agrawal, Ved Varun, Pandey, M K, Sumana, Gajjala
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2021
Zugriff auf das übergeordnete Werk:Langmuir : the ACS journal of surfaces and colloids
Schlagworte:Journal Article Graphite 7782-42-5
LEADER 01000naa a22002652 4500
001 NLM328241490
003 DE-627
005 20231225202537.0
007 cr uuu---uuuuu
008 231225s2021 xx |||||o 00| ||eng c
024 7 |a 10.1021/acs.langmuir.1c00896  |2 doi 
028 5 2 |a pubmed24n1094.xml 
035 |a (DE-627)NLM328241490 
035 |a (NLM)34278796 
040 |a DE-627  |b ger  |c DE-627  |e rakwb 
041 |a eng 
100 1 |a Solanki, Shipra  |e verfasserin  |4 aut 
245 1 0 |a Ultrasensitive Immunosensor Based on Langmuir-Blodgett Deposited Ordered Graphene Assemblies for Dengue Detection 
264 1 |c 2021 
336 |a Text  |b txt  |2 rdacontent 
337 |a ƒaComputermedien  |b c  |2 rdamedia 
338 |a ƒa Online-Ressource  |b cr  |2 rdacarrier 
500 |a Date Completed 02.08.2021 
500 |a Date Revised 02.08.2021 
500 |a published: Print-Electronic 
500 |a Citation Status MEDLINE 
520 |a In this manuscript partially reduced graphene oxide (RGO) nanosheet-based electrodes have been utilized for quantification of the NS1 protein and subsequently for dengue detection. NS1 is the biomarker found circulating in the body of dengue-infected persons on or after first day of the appearance of disease symptoms. Graphene oxide (GO) has been synthesized using a modified Hummer's method, and its ordered nanostructured films have been electrophoretically deposited on indium tin oxide (ITO)-coated glass substrates using Langmuir-Blodgett (LB) deposition. Deposited LB films of GO have been reduced with hydrazine vapors to obtain RGO-coated ITO electrodes. NS1 antibodies have been grafted onto the ordered thin films using covalent linking, and the bioelectrodes have been utilized for the specific detection of NS1 antigen. The electrochemical performance of the fabricated bioelectrodes for NS1 antigen detection has been explored in standard and spiked sera samples. The limit of detection for the standard samples and spiked serum samples is found to be 0.069 ng mL-1 and 0.081 ng mL-1, respectively, with a sensitivity of 8.41 and 36.75 Ω per ng mL, respectively, in the detection range of 101 to 107 ng mL-1 
650 4 |a Journal Article 
650 7 |a Graphite  |2 NLM 
650 7 |a 7782-42-5  |2 NLM 
700 1 |a Soni, Amrita  |e verfasserin  |4 aut 
700 1 |a Agrawal, Ved Varun  |e verfasserin  |4 aut 
700 1 |a Pandey, M K  |e verfasserin  |4 aut 
700 1 |a Sumana, Gajjala  |e verfasserin  |4 aut 
773 0 8 |i Enthalten in  |t Langmuir : the ACS journal of surfaces and colloids  |d 1992  |g 37(2021), 29 vom: 27. Juli, Seite 8705-8713  |w (DE-627)NLM098181009  |x 1520-5827  |7 nnns 
773 1 8 |g volume:37  |g year:2021  |g number:29  |g day:27  |g month:07  |g pages:8705-8713 
856 4 0 |u http://dx.doi.org/10.1021/acs.langmuir.1c00896  |3 Volltext 
912 |a GBV_USEFLAG_A 
912 |a SYSFLAG_A 
912 |a GBV_NLM 
912 |a GBV_ILN_22 
912 |a GBV_ILN_350 
912 |a GBV_ILN_721 
951 |a AR 
952 |d 37  |j 2021  |e 29  |b 27  |c 07  |h 8705-8713