|
|
|
|
| LEADER |
01000naa a22002652 4500 |
| 001 |
NLM328000531 |
| 003 |
DE-627 |
| 005 |
20231225202029.0 |
| 007 |
cr uuu---uuuuu |
| 008 |
231225s2021 xx |||||o 00| ||eng c |
| 024 |
7 |
|
|a 10.1111/nph.17615
|2 doi
|
| 028 |
5 |
2 |
|a pubmed24n1093.xml
|
| 035 |
|
|
|a (DE-627)NLM328000531
|
| 035 |
|
|
|a (NLM)34254310
|
| 040 |
|
|
|a DE-627
|b ger
|c DE-627
|e rakwb
|
| 041 |
|
|
|a eng
|
| 100 |
1 |
|
|a Greifenhagen, Anne
|e verfasserin
|4 aut
|
| 245 |
1 |
4 |
|a The Phtheirospermum japonicum isopentenyltransferase PjIPT1a regulates host cytokinin responses in Arabidopsis
|
| 264 |
|
1 |
|c 2021
|
| 336 |
|
|
|a Text
|b txt
|2 rdacontent
|
| 337 |
|
|
|a ƒaComputermedien
|b c
|2 rdamedia
|
| 338 |
|
|
|a ƒa Online-Ressource
|b cr
|2 rdacarrier
|
| 500 |
|
|
|a Date Completed 26.10.2021
|
| 500 |
|
|
|a Date Revised 26.10.2021
|
| 500 |
|
|
|a published: Print-Electronic
|
| 500 |
|
|
|a Citation Status MEDLINE
|
| 520 |
|
|
|a © 2021 The Authors. New Phytologist © 2021 New Phytologist Foundation.
|
| 520 |
|
|
|a The hemiparasitic plant Phtheirospermum japonicum (Phtheirospermum) is a nutritional specialist that supplements its nutrient requirements by parasitizing other plants through haustoria. During parasitism, the Phtheirospermum haustorium transfers hypertrophy-inducing cytokinins (CKs) to the infected host root. The CK biosynthesis genes required for haustorium-derived CKs and the induction of hypertrophy are still unknown. We searched for haustorium-expressed isopentenyltransferases (IPTs) that catalyze the first step of CK biosynthesis, confirmed the specific expression by in vivo imaging of a promoter-reporter, and further analyzed the subcellular localization, the enzymatic function and contribution to inducing hypertrophy by studying CRISPR-Cas9-induced Phtheirospermum mutants. PjIPT1a was expressed in intrusive cells of the haustorium close to the host vasculature. PjIPT1a and its closest homolog PjIPT1b located to the cytosol and showed IPT activity in vitro with differences in substrate specificity. Mutating PjIPT1a abolished parasite-induced CK responses in the host. A homolog of PjIPT1a also was identified in the related weed Striga hermonthica. With PjIPT1a, we identified the IPT enzyme that induces CK responses in Phtheirospermum japonicum-infected Arabidopsis roots. We propose that PjIPT1a exemplifies how parasitism-related functions evolve through gene duplications and neofunctionalization
|
| 650 |
|
4 |
|a Journal Article
|
| 650 |
|
4 |
|a Research Support, Non-U.S. Gov't
|
| 650 |
|
4 |
|a Striga
|
| 650 |
|
4 |
|a cytokinin
|
| 650 |
|
4 |
|a haustorium
|
| 650 |
|
4 |
|a hypertrophy
|
| 650 |
|
4 |
|a intrusive cells
|
| 650 |
|
4 |
|a neofunctionalization
|
| 650 |
|
4 |
|a parasite
|
| 650 |
|
7 |
|a Cytokinins
|2 NLM
|
| 650 |
|
7 |
|a Alkyl and Aryl Transferases
|2 NLM
|
| 650 |
|
7 |
|a EC 2.5.-
|2 NLM
|
| 650 |
|
7 |
|a adenylate isopentenyltransferase
|2 NLM
|
| 650 |
|
7 |
|a EC 2.5.1.27
|2 NLM
|
| 700 |
1 |
|
|a Braunstein, Isabell
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Pfannstiel, Jens
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Yoshida, Satoko
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Shirasu, Ken
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Schaller, Andreas
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Spallek, Thomas
|e verfasserin
|4 aut
|
| 773 |
0 |
8 |
|i Enthalten in
|t The New phytologist
|d 1979
|g 232(2021), 4 vom: 15. Nov., Seite 1582-1590
|w (DE-627)NLM09818248X
|x 1469-8137
|7 nnns
|
| 773 |
1 |
8 |
|g volume:232
|g year:2021
|g number:4
|g day:15
|g month:11
|g pages:1582-1590
|
| 856 |
4 |
0 |
|u http://dx.doi.org/10.1111/nph.17615
|3 Volltext
|
| 912 |
|
|
|a GBV_USEFLAG_A
|
| 912 |
|
|
|a SYSFLAG_A
|
| 912 |
|
|
|a GBV_NLM
|
| 912 |
|
|
|a GBV_ILN_350
|
| 951 |
|
|
|a AR
|
| 952 |
|
|
|d 232
|j 2021
|e 4
|b 15
|c 11
|h 1582-1590
|