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231225s2021 xx |||||o 00| ||eng c |
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|a 10.1094/PDIS-04-21-0688-RE
|2 doi
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|a pubmed24n1224.xml
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|a eng
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|a Li, Changfeng
|e verfasserin
|4 aut
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|a Development of Recombinase Polymerase Amplification Combined with Lateral Flow Detection Assay for Rapid and Visual Detection of Ralstonia solanacearum in Tobacco
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|c 2021
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
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|a ƒa Online-Ressource
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|a Date Completed 25.01.2022
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|a Date Revised 13.12.2023
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a Bacterial wilt caused by Ralstonia solanacearum is a serious soilborne disease that results in severe losses to tobacco (Nicotiana tabacum) production in China. In this study, a novel RPA-LFD assay for the rapid visual detection of R. solanacearum was established using recombinase polymerase amplification (RPA) and lateral-flow dipstick (LFD). The RPA-LFD assay was performed at 37°C in 30 min without complex equipment. Targeting the sequence of the RipTALI-9 gene, we designed RPA primers (Rs-rpa-F/R) and an LF probe (Rs-LF-probe) that showed high specificity to R. solanacearum. The sensitivity of RPA-LFD assay to R. solanacearum was the same as that in conventional PCR at 1 pg genomic DNA, 103 CFU/g artificially inoculated tobacco stems, and 104 CFU/g artificially inoculated soil. The RPA-LFD assay could also detect R. solanacearum from plant and soil samples collected from naturally infested tobacco fields. These results suggest that the RPA-LFD assay developed in this study is a rapid, accurate molecular diagnostic tool with high sensitivity for the detection of R. solanacearum
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|a Journal Article
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|a Ralstonia solanacearum
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|a molecular diagnosis
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| 650 |
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|a recombinase polymerase amplification
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| 650 |
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4 |
|a sensitivity
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|a specificity
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| 650 |
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|a Recombinases
|2 NLM
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1 |
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|a Ju, Yuliang
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Shen, Pengfei
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Wu, Xun
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Cao, Le
|e verfasserin
|4 aut
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| 700 |
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|a Zhou, Benguo
|e verfasserin
|4 aut
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| 700 |
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|a Yan, Xiaoming
|e verfasserin
|4 aut
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| 700 |
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|a Pan, Yuemin
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Plant disease
|d 1997
|g 105(2021), 12 vom: 22. Dez., Seite 3985-3989
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|g volume:105
|g year:2021
|g number:12
|g day:22
|g month:12
|g pages:3985-3989
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|u http://dx.doi.org/10.1094/PDIS-04-21-0688-RE
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