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231225s2021 xx |||||o 00| ||eng c |
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|a 10.2144/btn-2021-0051
|2 doi
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|a pubmed25n1092.xml
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|e rakwb
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|a eng
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| 100 |
1 |
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|a 'Giron' Koetsier, Paul A
|e verfasserin
|4 aut
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| 245 |
1 |
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|a A simple approach for effective shearing and reliable concentration measurement of ultra-high-molecular-weight DNA
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|c 2021
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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|a Date Completed 20.12.2021
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|a Date Revised 20.12.2021
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a Pipetting and concentration measurement of viscous ultra-high-molecular-weight (UHMW) DNA samples is challenging and often highly imprecise. Effective guidelines for handling UHMW samples are missing in the field. Herein, a simple and low-cost workflow is presented that enables accurate pipetting and reliable concentration measurement. Central to the workflow is the shearing of representative small aliquots of UHMW DNA samples to a fragment size <150 kb by vortexing them for 1 min with a glass bead in a round-bottomed 2-ml tube. Additionally, a solution is provided for accurate quantitation of high-molecular-weight DNA with fluorometric (Qubit [Thermo Fisher Scientific, MA, USA]) methods by using an appropriate genomic DNA standard, resulting in values that match spectrophotometric (Nanodrop [Thermo Fisher Scientific]) optical density readings
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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4 |
|a DNA extraction
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| 650 |
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4 |
|a DNA shearing
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| 650 |
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4 |
|a genomics
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| 650 |
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4 |
|a glass beads
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| 650 |
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4 |
|a nanodrop
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| 650 |
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4 |
|a needle shearing
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| 650 |
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4 |
|a quantification
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| 650 |
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4 |
|a qubit
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| 650 |
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4 |
|a ultra-high-molecular-weight DNA
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| 650 |
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|a DNA
|2 NLM
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| 650 |
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7 |
|a 9007-49-2
|2 NLM
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| 700 |
1 |
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|a Cantor, Eric J
|e verfasserin
|4 aut
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| 773 |
0 |
8 |
|i Enthalten in
|t BioTechniques
|d 1991
|g 71(2021), 2 vom: 03. Aug., Seite 439-444
|w (DE-627)NLM012627046
|x 1940-9818
|7 nnas
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| 773 |
1 |
8 |
|g volume:71
|g year:2021
|g number:2
|g day:03
|g month:08
|g pages:439-444
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| 856 |
4 |
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|u http://dx.doi.org/10.2144/btn-2021-0051
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