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231225s2021 xx |||||o 00| ||eng c |
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|a 10.1111/cobi.13802
|2 doi
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|a pubmed24n1092.xml
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|a (DE-627)NLM327707755
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|a (NLM)34224158
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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| 100 |
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|a Marques, Virginie
|e verfasserin
|4 aut
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| 245 |
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|a Use of environmental DNA in assessment of fish functional and phylogenetic diversity
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|c 2021
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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| 338 |
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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|a Date Completed 27.01.2022
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|a Date Revised 27.01.2022
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a © 2021 Society for Conservation Biology.
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|a Assessing the impact of global changes and protection effectiveness is a key step in monitoring marine fishes. Most traditional census methods are demanding or destructive. Nondisturbing and nonlethal approaches based on video and environmental DNA are alternatives to underwater visual census or fishing. However, their ability to detect multiple biodiversity factors beyond traditional taxonomic diversity is still unknown. For bony fishes and elasmobranchs, we compared the performance of eDNA metabarcoding and long-term remote video to assess species' phylogenetic and functional diversity. We used 10 eDNA samples from 30 L of water each and 25 hr of underwater videos over 4 days on Malpelo Island (pacific coast of Colombia), a remote marine protected area. Metabarcoding of eDNA detected 66% more molecular operational taxonomic units (MOTUs) than species on video. We found 66 and 43 functional entities with a single eDNA marker and videos, respectively, and higher functional richness for eDNA than videos. Despite gaps in genetic reference databases, eDNA also detected a higher fish phylogenetic diversity than videos; accumulation curves showed how 1 eDNA transect detected as much phylogenetic diversity as 25 hr of video. Environmental DNA metabarcoding can be used to affordably, efficiently, and accurately census biodiversity factors in marine systems. Although taxonomic assignments are still limited by species coverage in genetic reference databases, use of MOTUs highlights the potential of eDNA metabarcoding once reference databases have expanded
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|a Journal Article
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| 650 |
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4 |
|a Research Support, Non-U.S. Gov't
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| 650 |
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|a Malpelo
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| 650 |
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|a accumulation curves
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| 650 |
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|a arrecifes tropicales
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| 650 |
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|a biodiversidad
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|a biodiversity
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| 650 |
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|a caracterización genética con ADNa
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| 650 |
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|a características funcionales
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| 650 |
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|a curvas de acumulación
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| 650 |
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|a eDNA metabarcoding
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| 650 |
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|a functional traits
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| 650 |
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|a marine protected area
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| 650 |
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|a tropical reefs
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| 650 |
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|a video
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| 650 |
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|a área marina protegida
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| 650 |
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|a 功能性状
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| 650 |
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|a 海洋保护区
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| 650 |
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|a 热带珊瑚礁
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| 650 |
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|a 环境DNA宏条形码
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| 650 |
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|a 生物多样性
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| 650 |
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|a 累积曲线
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| 650 |
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|a 视频
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|a 马尔佩洛岛
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| 650 |
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|a DNA, Environmental
|2 NLM
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1 |
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|a Castagné, Paul
|e verfasserin
|4 aut
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| 700 |
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|a Polanco, Andréa
|c Fernández
|e verfasserin
|4 aut
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| 700 |
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|a Borrero-Pérez, Giomar Helena
|e verfasserin
|4 aut
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|a Hocdé, Régis
|e verfasserin
|4 aut
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|a Guérin, Pierre-Édouard
|e verfasserin
|4 aut
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|a Juhel, Jean-Baptiste
|e verfasserin
|4 aut
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|a Velez, Laure
|e verfasserin
|4 aut
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|a Loiseau, Nicolas
|e verfasserin
|4 aut
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|a Letessier, Tom Bech
|e verfasserin
|4 aut
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|a Bessudo, Sandra
|e verfasserin
|4 aut
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|a Valentini, Alice
|e verfasserin
|4 aut
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|a Dejean, Tony
|e verfasserin
|4 aut
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|a Mouillot, David
|e verfasserin
|4 aut
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|a Pellissier, Loïc
|e verfasserin
|4 aut
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| 700 |
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|a Villéger, Sébastien
|e verfasserin
|4 aut
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| 773 |
0 |
8 |
|i Enthalten in
|t Conservation biology : the journal of the Society for Conservation Biology
|d 1999
|g 35(2021), 6 vom: 05. Dez., Seite 1944-1956
|w (DE-627)NLM098176803
|x 1523-1739
|7 nnns
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| 773 |
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|g volume:35
|g year:2021
|g number:6
|g day:05
|g month:12
|g pages:1944-1956
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|u http://dx.doi.org/10.1111/cobi.13802
|3 Volltext
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|d 35
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|h 1944-1956
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